[关键词]
[摘要]
目的 研究并建立黔产辣蓼Polygonum hydropiper药材指纹图谱。方法 采用HPLC法,Hypersil ODS2 C18柱,以乙腈-0.2%磷酸水溶液为流动相进行梯度洗脱,检测波长为260 nm,柱温25℃,对22批不同产地辣蓼药材及其混淆品进行检测。结果 建立辣蓼药材HPLC指纹图谱共有模式,标定12个共有峰。并对15批不同产地辣蓼药材及其7批混淆品进行了相似度比较。15批辣蓼药材相似度均大于0.95,7批混淆品均小于0.50。结论 辣蓼药材HPLC指纹图谱具有重复性好、特征性强、方法简便等特点,可作为黔产辣蓼药材的识别方法。
[Key word]
[Abstract]
Objective To study and establish the fingerprints of polygonilterba the aerial pevrts of Polygonum hydropiper by HPLC. Methods The fingerprints of P. hydropiper from different origins were built using Hypersil ODS2 C18 column and acetonitrile-0.2%phosphoric acid as mobile phase. The flow rate was 1.0 mL/min. The detecting wavelength was set at 260 nm. The temperature of column was at 25℃. Total 22 Polygonum Herba samples and adulterants were detected. Results The mutual mode to HPLC fingerprints was set up, and the 12 common peaks were obtained. The similarities in 15 batches of Polygonum Herba samples were all above 0.95 and those of seven batches of adulterants were below 0.50. Conclusion The method is simple, stable, and reproducible, which can be uesd to identify Polygonum Herba and its adulterants.
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[基金项目]
贵州省科技合作专项资金项目(省市科合[2013]40号)