[关键词]
[摘要]
目的 比较六味地黄配方颗粒与传统汤剂6种有效成分的量。方法 采用HPLC法在不同色谱条件下测定六味地黄配方颗粒与传统汤剂中毛蕊花糖苷、马钱苷、丹皮酚、尿囊素、茯苓酸、23-乙酰泽泻醇B的量。结果 传统汤剂中毛蕊花糖苷、马钱苷、丹皮酚、尿囊素、茯苓酸和23-乙酰泽泻醇B的质量分数分别为304.5、2473.6、3135.1、708.8、5.9、104.4μg/g,对应配方颗粒分别为298.6、3685.7、706.5、714.2、17.4、217.8μg/g;传统汤剂与对应配方颗粒中毛蕊花糖苷和尿囊素的量基本一致;马钱苷、茯苓酸和23-乙酰泽泻醇B的量,配方颗粒明显高于传统饮片;丹皮酚的量配方颗粒明显低于传统饮片。结论 配方颗粒与传统汤剂中化学成分量的高低与其组分化学性质有关。
[Key word]
[Abstract]
Objective To compare the content of chemical components in each drug in formula granule and traditional decoction of Rehmanniae Decoction with six ingredients. Methods By using high performance liquid chromatography analysis and various chromatographic conditions, the contents were determined respectively, which were listed as follows: acteoside, loganin, paeonol, allantoin, pachymic acid and alisol B 23-acetate. Results The contents of acteoside, loganin, paeonol, allantoin, pachymic acid and alisol B 23-acetate in traditional decoction were 304.5, 2 473.6, 3 135.1, 708.8, 5.9, and 104.4 μg/g, and they were 289.6, 3 685.7, 706.5, 714.2, 17.4, and 217.8 μg/g correspondingly in formula granule. The contents of acteoside and allantoin were basically the same between them; The contents of loganin, pachymic acid, and alisol B 23-acetate in formula granule were significantly higher than those in the traditional decoction; The content of paeonol in formula granule was significantly lower than that in the traditional decoction. Conclusion The content difference of the chemical components is related to its chemical character between formula granule and traditional decoction.
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[基金项目]
天津市科技支撑计划重点项目,生物医药领域(12ZCZDSY01000)——"天津市配方颗粒质量标准研究"