目的 研究经典复方平胃散拆方后臣药厚朴主要化学成分厚朴酚与和厚朴酚煎出量的变化。方法 将经典复方平胃散以臣药厚朴为基础进行拆方分析,主要分为11个组别(单味药组别1个、复方组别4个、以厚朴为主的组别6个)进行分析。利用反相高效液相色谱法进行检测,GRACE AlltimaTM C₁₈色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-水,等度洗脱,柱温25℃,检测波长294 nm,体积流量1 mL/min。结果 和厚朴酚在20.12~120.72μg/mL(r=0.9990),厚朴酚在20.16~120.96μg/mL(r=0.9991)线性关系良好。精密度和重复性考察中,和厚朴酚与厚朴酚的RSD值均小于2%,表明仪器的精密度及方法的重复性良好。稳定性试验表明各测定成分在24 h内稳定性良好。加样回收试验中和厚朴酚、厚朴酚平均回收率分别为98.69%、101.28%,RSD值分别为1.47%、1.24%,表明方法的准确性良好。君药苍术、生姜、大枣可以使复方中厚朴酚与和厚朴酚在平胃散复方中煎出量降低;佐药陈皮、使药甘草可以使厚朴酚与和厚朴酚在平胃散复方中煎出量增加。结论 厚朴酚与和厚朴酚在平胃散复方中的量受方中其他药物的影响,可以从一定程度上说明平胃散复方配伍与减毒增效的相关性,为后续研究提供一定的参考。

Objective To develop an HPLC-DAD method for the detemination of the content change of honokiol and magnolol from classical Pingweisan (a prescription of Chinese materia medica used for stomach discomfort), and it is based on minister (Chen) medicine compatibility. Methods After the decomposition of classical Pingweisan besed on Magnoliae Officinalis Cortex as minister medicine, 11 groups (one individual herb group, four compound groups, six groups with Magnoliae Officinalis Cortex as main medicine) were divided. The analysis was carried out on a GRACE AlltimaTM C₁₈ (250 mm×4.6 mm, 5 μm) column with a mobile phase which was composed of acetonitrile (B) and water (A) for isocratic elution. The column temperature was 25℃, the flow rate was 1 mL/min, and the detection wavelength was set at 294 nm. Results The linear relationship of honokiol and magnolol is better within the ranges in 20.12-120.72 μg/mL (r=0.999 0) and 20.16-120.96 μg/mL (r=0.999 1); Study on the precision and repeatability, the RSD value of both honokiol and magnolol was less than 2%, which showed that the precision of the instrument and the method of repeatability were favourable. Stability test showed that the determination of the components was stable within 24 h. The recoveries were 98.69% and 101.28%, and the RSD values were 1.47% and 1.24%, which showed that the method had a good accuracy. Monarch Atractylodis Rhizoma, Zingberis, and Jujubae Fructus could make the contents of honokiol and magnolol in Pingweisan reduced; while the assistant Citri Reticulatae Pericarpium and guiding Glycyrrhizae Radix could make the contents increased. Conclusion The other crude drugs in Pingweisan could affect and change the contents of honokiol and magnolol. It could explain the synergistic effect of compatibility and detoxification of the classical compound Pingweisan.

国家自然科学基金资助项目(81373544)