[关键词]
[摘要]
目的 对生脉注射液生产过程五味子药渣中多种类型可利用资源性化学成分进行分析与评价,以期为五味子药材深加工产业化过程的循环利用提供科学依据。方法 对五味子药渣采用HPLC-UV法对木脂素类成分进行分析;采用碱液法提取蛋白质类成分,BCA法以牛血清白蛋白为对照测定总蛋白质质量分数;采用紫外-可见分光光度计测定中性多糖和酸性多糖的组成及质量分数;采用FIWE3/6纤维测定法测定粗纤维质量分数。结果 研究表明,五味子药渣中五味子甲素、五味子乙素、五味子丙素、五味子醇甲、五味子醇乙和五味子酯甲的质量分数依次为1.4424、3.7880、1.3509、4.3993、3.2313、0.5053 mg/g;与原药材相比,在生脉注射液生产过程中五味子醇甲的提取利用率为20.84%;而五味子醇乙几乎未被利用而残留于药渣中;五味子甲素、五味子乙素、五味子丙素和五味子酯甲在药渣中的质量分数略高于原药材。五味子药渣中可利用生物大分子类物质分别为总蛋白质质量分数为14.69%;中性多糖质量分数为3.82%,酸性多糖质量分数为1.31%;粗纤维素类成分质量分数为43.80%。结论 通过分析表明生脉注射液生产过程产生的五味子药渣中尚含有丰富的木脂素类、蛋白质类、多糖类及粗纤维素类等资源性化学物质,针对水提工艺产生的五味子药渣中可利用资源性化学物质探索性地提出了其循环利用对策与可能途径,为中药资源深加工产业化过程废弃物的资源化利用提供借鉴,为提倡和推进资源节约和环境保护发展,实现经济和生态和谐共生提供参考。
[Key word]
[Abstract]
Objective Multi-type resource chemical constituents in Schizandrae Fructus residues were analyzed in the process of Shengmai Injection production, in order to provide the scientific basis for Schizandrae Fructus in the further process of industrialization. Methods The lignan components were analysed and evaluated by HPLC-UV method. After using NaOH to extract sample, the BCA method was adopted to measure the mass fraction of total protein and take bovine serum albumin as the reference. Using UV-Vis spectrophotometer to measure the mass fraction and constitutes of neutral polysaccharide and acidic polysaccharide, respectively. The equipment for raw fiber determination was taken to gauge the crude fiber content of Schizandrae Fructus residues. Results The mass fractions of schisandrin A, schizandrin B, schizandrin C, gomisin A, gomisin B, and schisantherin A were 1.442 4, 3.788 0, 1.350 9, 4.399 3, 3.231 3, and 0.505 3 mg/g, respectively. Compared with the original medicinal materials, the technology utilization rate of gomisin A was 20.84% during the process of Shengmai Injection production. But the gomisin B virtually was unused and remained in the residues. The mass fractions of schisandrin A, schizandrin B, schizandrin C, and schisantherin A were higher than that of the original medicinal material. The available macromolecular substances are proteins, polysaccharide, and crude fiber. The content of total proteins was 14.69%. The mass fractions of neutral polysaccharide and acidic polysaccharide were 3.82% and 1.31%, respectively. The analysis on crude fiber showed that the mass fraction of crude fiber in Schizandrae Fructus residues was 43.80%. Conclusion The analysis shows that Schizandrae Fructus residues contain many resource chemical components in the process of Shengmai Injection production, such as lignins, protein, saccharides, and crude fiber. The strategy for recycling and possible way is proposed exploringly based on the available resource chemical components of Schizandrae Fructus residues and water-extracting technology. It provides the reference for the utilization of waste resource in further process of industralization, promoting the resource conservation, and development of environmental protection, realizing the harmonious coexistence between the economy and ecology.
[中图分类号]
[基金项目]
江苏高校中药资源产业化过程协同创新中心建设专项(2013年度);江苏省"333高层次人才工程"第一层次培养对象资助项目(2013年度);江苏省高校中药学优势学科II期建设项目(ysxk-2014)