[关键词]
[摘要]
目的 研究当归Angelica sinensis干燥根的化学成分。方法 运用硅胶、凝胶、MCI-Gel树脂及RP-HPLC等多种色谱技术分离纯化,并根据理化性质和波谱数据鉴定化合物的结构。结果 从当归干燥根95%乙醇提取物中分离得到了1个新香豆素类化合物,鉴定为6-羟基-3-(4-羟基苯)-7-甲基-香豆素(1);化合物1对A549和MCF-7细胞株IC50值分别为3.2和2.8μmol/L。结论 化合物1为1个新的香豆素类化合物,命名为云归香豆素A;其对A549和MCF-7细胞株表现了明显的体外细胞毒活性。
[Key word]
[Abstract]
Objective To study the chemical constituents from the roots of Angelica sinensis. Methods The chemical constituents in the roots of A. sinensis were separated and purified through various kinds of chromatographic methods, including silica gel, MCI-Gel, Sephadex LH-20, and RP-HPLC. Their structures were elucidated by spectral data and physical-chemical properties. Results 6-Hydroxy-3-(4-hydroxyphenyl)-7-methyl-coumarin (1) was isolated from the 95% ethanol extracts of this plant. Conclusion Compound 1 is a new compound, named Angelica coumarin A. And it displays the cytotoxicity against A549 and MCF7 cells with IC50 values of 3.2 and 2.8 μmol/L, respectively.
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[基金项目]
国家自然科学基金资助项目(21362044,21032085);云南省高校科技创新团队资助项目(IRTSTYN2014-11)