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[摘要]
目的 对广金钱草Desmodium styracifolium种子的化学成分及其清除DPPH自由基活性进行研究。方法 采用硅胶、聚酰胺、Sephadex LH-20凝胶柱色谱等色谱方法进行分离纯化,通过NMR、MS等波谱数据进行结构鉴定。比较不同极性提取物总黄酮的量及其对DPPH自由基的清除活性。结果 从广金钱草种子的不同极性萃取部位中分离得到10个化合物,分别鉴定为豆甾醇(1)、邻苯二甲酸二正丁酯(2)、儿茶素(3)、苯甲酸(4)、麦芽酚(5)、3-O-乙酰基-(-)-表儿茶素(6)阿福豆苷(7)、3'-甲氧基-表儿茶素(8)、山柰酚-7-O-β-D-吡喃葡萄糖苷(9)、槲皮素-3-O-β-D-吡喃葡萄糖苷(10)。结论 其中化合物4~6、8为首次从山蚂蝗属植物中分离得到,化合物2、3、7、9为首次从广金钱草中分离得到。广金钱草种子的各段萃取物均表现出一定的DPPH自由基清除活性。
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[Abstract]
Objective To study the chemical constituents from the seeds of Desmodium styracifolium and their activity of scavenging DPPH free radicals. Methods The compounds were isolated and purified by various column chromatographyic techniques (silica gel, polyamide, sephadex LH-20, etc) and their structures were elucidated by MS and NMR spectral analyses. The activities of scavenging DPPH free radicals from different extracts from the seeds of D. styracifolium were compared. Results Ten compounds were isolated and identified from the seeds of D. styracifolium, including stigmasterol (1), dibutyl phthalate (2), (+)-catechin (3), benzoic acid (4), 3-hydroxy-2-methyl-4-pyrone (5), 3-O-acetyl (-)-epicatechin (6), afzelin (7), 3'-O-methyl epicatechin (8), kaempferol-7-O-β-D-glucopyranoside (9), and quercetin-3-O-β-D-glucopyranoside (10). Conclusion Compounds 4-6 and 8 are isolated from the plants of Desmodium Desv. for the first time. Compounds 2, 3, 7, and 9 are isolated from D. styracifolium for the first time. The different extracts from the seeds of D. styracifolium exhibit the certain activity of scavenging DPPH free radicals.
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