目的 研究炮制辅料羊脂油促进淫羊藿总黄酮肠吸收转运机制。方法 采用大鼠在体单向肠灌流模型和Caco-2细胞单层模型研究炮制辅料羊脂油对淫羊藿总黄酮自组装形成胶束后肠吸收的影响。结果 大鼠在体单向肠灌流模型中, 淫羊藿总黄酮中淫羊藿苷成分在4个肠段的吸收具有差异性, 其中在空肠段最高。加入羊脂油自组装形成胶束后, 在十二指肠和空肠段的渗透系数显著增加。Caco-2细胞单层模型中, 淫羊藿总黄酮中的淫羊藿苷成分吸收渗透系数较小, 加入羊脂油自组装形成胶束后吸收渗透系数显著增加, 外排比率从4.72下降到了2.31。结论 淫羊藿总黄酮肠吸收较差, 加入羊脂油后可自组装形成胶束, 其在肠道的吸收增加。

Objective To study the promotion mechanism of suet oil to the intestinal absorption of the total flavonoids from Epimedii Folium (EF). Methods The in vivo intestinal perfusion model and Caco-2 cell monolayer model of rats were used to study the influence of processing excipient suet oil in self-assembled micelles on the intestinal absorption of total flavones from EF. Results In the in vivo intestinal perfusion model of rats, there were differences of absorption of icariin in the total flavonoids from EF in the four segments of the intestines. After the suet oil was added and self-assembled micelles were formed, the permeabilities of icariin in the total flavonoids from EF were increased significantly in duodenum and jejunum segments. In Caco-2 cell monolayer model of rats, the absorptive permeability coefficients were small for icariin in the total flavonoids from EF, after the suet oil was added and the self-assembled micelles were formed, the absorptive permeability coefficients were increased significantly, the efflux ratios were decreased from 4.72 to 2.31. Conclusion The total flavonoids from EF have a bad intestinal absorption, after the suet oil added and the self-assembled micelles formed, the intestinal absorption of the total flavonoids from EF could be improved.

国家自然科学基金资助项目(30572372, 30973944, 81274088)