目的 建立五味子咀嚼片的HPLC-DAD指纹图谱, 对10个不同批次的五味子咀嚼片进行质量评价。方法 使用Luna C₁₈色谱柱(250 mm×4.6 mm, 5 μm), 乙腈-0.05%磷酸水溶液为流动相进行梯度洗脱, 体积流量1.0 mL/min, 柱温35 ℃, 检测波长220 nm, 进样量20 μL。结果 通过测定10批五味子咀嚼片的HPLC-DAD图谱, 建立了指纹图谱的共有模式, HPLC-DAD指纹图谱共有峰39个, 相似度为0.914～0.999;确认了五味子醇甲、五味子醇乙、五味子酯甲、五味子乙素、五味子丙素、丹参素钠、丹酚酸B、隐丹参酮、丹参酮II_A、原儿茶醛、木犀草苷、蒙花苷。结论 方法准确、可靠、重现性好, 为评价五味子咀嚼片的质量提供了科学依据。

Objective To establish the chromatography fingerprint of Schisandra chinensis Chewable Tablets (SCCT) with HPLC-DAD and to evaluate SCCT from 10 different batches. Methods Luna C₁₈ column (250 mm × 4.6 mm, 5 μm) was used, mobile phase was acetonitrile-0.05% phosphate, flow speed was 1.0 mL/min, temperature of column was set at 35 ℃, detected wavelength was at 220 nm, and injection volume was 20 μL. Results The chromatography fingerprint of SCCT from 10 different batches was established. In the chromatography fingerprint with HPLC-DAD of SCCT, 39 common peaks were demarcated and the similarities of SCCT were between 0.914—0.999. Schizandrin, schisandrol B, schisantherin A, schisandrin B, schisandrin C, salvianic acid A sodium, salvianolic acid B, cryptotanshinone, tanshinone II_A, 3,4-dihydroxyhenzaldehyde, luteoloside, and buddleoside were identified. Conclusion The method is accurate, reliable and with good reproducibility, and can be used as the evidence for the quality evaluation of SCCT.

吉林省科技厅项目(20130727084YY)