目的 中试规模下优化微波提取延胡索Corydalis Rhizoma中有效成分(延胡索乙素)的最佳工艺。方法 通过单因素试验考察药材炮制、微波功率、溶媒浓度、液固比、提取时间等因素对延胡索乙素提取率的影响, 并在此基础上通过Box-Behnken法优化延胡索微波提取工艺。结果 延胡索微波提取的最佳工艺为以水为提取溶媒, 微波功率7 kW, 液固比10:1, 提取21 min, 在此条件下延胡索乙素提取率预测值为83.60%, 验证值为83.74%;且与煎煮提取相比, 干浸膏得率降低70%。结论 所建立的数学模型精度高(P<0.01), 可对延胡索微波提取工艺进行分析和预测。

Objective To optimize the conditions of microwave technique in extraction of effective ingredients in Corydalis Rhizoma in the condition of pilot scale. Methods Medicinal materials processing, microwave power, solvent concentration, liquid to solid ratio, extraction time and so on were investigated by single factor test on the extraction rate of tetrahydropalmatine, and on this basis, using the method of Box-Behnken the microwave extraction process of Corydalis Rhizoma was optimized. Results The optimum process of microwave extraction of Corydalis Rhizoma was as follows: using drinking water as the extraction solvent, microwave power of 7 kW, liquid to solid ratio of 10:1, and extraction for 21 min. Under these conditions, the extraction rate of tetrahydropalmatine predictive value was 83.60%, and the verified value was 83.74%; and compared with water extraction,dry extract rate decreased by 70%. Conclusion The mathematical model established is significant (P < 0.01) and can analyze and forecast the microwave extraction technology of Corydalis Rhizoma.

科学技术部国家“重大新药创制”项目(2013ZX09402203)