[关键词]
[摘要]
目的 研究苦丁茶冬青Ilex kudingcha根的化学成分。方法 采用硅胶柱色谱、Sephadex LH-20、制备液相等多种色谱技术进行化合物的分离纯化, 根据理化性质和波谱数据鉴定化合物的结构。结果 从苦丁茶冬青根95%乙醇提取物中分离得到19个化合物, 分别鉴定为α-香树醇-3β-棕榈酸酯(1)、β-谷甾醇(2)、α-香树精(3)、3-乙酰氧基-12-齐墩果烯-28-醇(4)、3-乙酰氧基-12-乌苏烯-28-醇(5)、3-乙酰氧基-12-乌苏烯-28-醛(6)、齐墩果酸(7)、乌苏酸(8)、槲皮素(9)、胡萝卜苷(10)、ficuscarpanoside B(11)、3β-O-28-去甲-12,17(18)-二烯乌苏烷(12)、逞罗树脂酸(13)、坡模酸((14)、α-D-葡萄糖(15)、2,6-二甲氧基苯醌(16)、丁香醛(17)、邻苯二甲酸二丁酯(18)、ilexoside D(19)。结论 化合物6、12、16~18为首次从该植物中分离得到, 化合物11为首次从该属植物中分离得到。
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[Abstract]
Objective To investigate the chemical constituents in the roots of Ilex kudingcha. Methods Column chromatography such as silica gel, Sephadex LH-20, and preparative HPLC were used to isolate and purify the compounds. Spectroscopic methods like MS, 1H-NMR, and 13C-NMR, and physical constants were used to elucidate their structures. Results Nineteen compounds were isolated from 95% ethanol extracts of I. kudingcha, including α-aromadendrol-3β-palmitate (1), β-sitosterol (2), α-amyrin (3), 3-acetoxyolean-12-en-28-ol (4), 3-acetoxyurs-12-en-28-ol (5), 3β-acetoxyurs-12-en-28-al (6), oleanolic acid (7), ursolic acid (8), quercetin (9), daucosterol (10), ficuscarpanoside B (11), 3β-hydroxy-28-norurs-12,17-dien (12), siaresinolic acid (13), pomolic acid (14), α-D-glucose (15), 2,6-dimethoxy-benzoquinone (16), syringaldehyde (17), dibutyl phthalate (18), and ilexoside D (19). Conclusion Compounds 6, 12, 16—18 are isolated from the plant for the first time. Compound 11 is isolated from Ilex L. for the first time.
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[基金项目]
广西中药质量标准研究重点实验室系统性研究课题(桂科重201209);国家科技支撑计划(2011BAI01B04);广西科学研究与技术开发计划(桂科重14124002-3)