目的 研究桦褐孔菌Inonotus obliquus子实体的化学成分。方法 采用硅胶、MCI、Sephadex LH-20凝胶等柱色谱方法进行分离纯化, 根据理化性质和波谱分析对化合物结构进行鉴定。结果 从桦褐孔菌子实体95%乙醇提取物中分离得到13个三萜化合物和2个甾醇化合物, 分别鉴定为3-羊毛甾-8,24-二烯-21-醛(1)、羊毛甾醇(2)、3β-羟基-羊毛甾-8,24-二烯-21-醛(3)、白桦脂醇(4)、桦褐孔菌醇(5)、栓菌酸(6)、3β,21-二羟基-羊毛甾-8,24-二烯(7)、齐墩果酸(8)、乌苏酸(9)、白桦脂酸(10)、桦褐孔菌素A(11)、桦褐孔菌萜D(12)、3β-乙酰氧基-11α,12α-环氧-齐墩果烷-28,13β-内酯(13)、麦角甾醇(14)、麦角甾烷-4,6,8,22-四烯-3-酮(15)。结论 化合物1为1个新的三萜化合物, 命名为桦褐孔菌素D;化合物9、13、15为首次从桦褐孔菌子实体中分离得到。

Objective To study the triterpenoids from the fruiting body of Inonotus obliquus. Methods The compounds were isolated and purified by silica gel, MCI gel, Sephadex LH-20 column chromatographies, and their structures were mainly elucidated on the basis of physicochemical characteristics and spectral analysis. Results Fifteen compounds were isolated from 95% EtOH extract of I. obliquus, and were identified as 3-oxo-lanosta-8,24-diene-21-al (1), lanosterol (2), 3β-hydroxy-lanosta-8,24-diene-21-al (3), betulin (4), inotodiol (5), trametenolic acid (6), 3β,21-dihydroxy-lanosta-8,24-diene (7), oleanic acid (8), ursolic acid (9), betulinic acid (10), inonotusane A (11), inoterpene D (12), 3-O-acetyl-11α,12α-epoxy-oleanan-28,13β-olide (13), ergosterol (14), and ergosta-4,6,8,22-tetraene-3-one (15), respectively. Conclusion Compound 1 is a new triterpenoid named as inonotusane D, while compounds 9, 13, and 15 are isolated from I. obliquus for the first time.

国家自然科学基金资助项目(81202902);四川省教育厅资助项目(15za0093)