目的 研究并建立不同产地冬凌草药材的HPLC-PDA指纹图谱, 并结合主成分分析法(PCA)评价不同产地的冬凌草药材质量, 为科学评价与有效控制冬凌草药材质量提供可靠的方法。方法 采用HPLC-PDA法构建冬凌草药材的指纹图谱, 同时应用PCA和相似度分析法对实验数据进行处理, 以找出4个不同产地18批冬凌草样品间的相似性及差异性。色谱柱为Diamonsil^TM C₁₈柱(250 mm×4.6 mm, 5 μm), 柱温为30 ℃;检测波长为238 nm, 流动相为乙腈-0.05%磷酸水进行梯度洗脱, 体积流量0.8 mL/min。结果 标定了冬凌草药材HPLC指纹图谱的19个共有峰, 并指认了其中的4个共有峰, 分别为冬凌草甲素、冬凌草乙素、拉西多宁和迷迭香酸, 18批冬凌草药材的相似度为0.421～0.984。PCA结果表明, 前3个因子的累积贡献率达到87.3%, 选择这3个因子对冬凌草药材进行综合评价。结论 HPLC指纹图谱结合PCA可以快速、客观地评价冬凌草药材的质量差异。

Objective To establish HPLC-PDA fingerprint for study on Rabdosiae Rubescentis Herba (RRH) from different areas and to scientifically evaluate and effectively control the quality of RRH from different areas by HPLC fingerprint combined with principal component analysis (PCA). Methods The fingerprint of RRH was established by HPLC-PDA and PCA and semblance analysis methods were applied to treating the experimental data in order to fine the similarity and difference among 18 batches of RRH from four different areas. The chromatographic procedure was carried out with Diamonsil^TM C₁₈ (250 mm × 4.6 mm, 5 μm) as an analytic column and a mixture consisting of acetonitrile-0.05% phosphoric acid in gradient as mobile phase, and the temperature of column was 30 ℃. The detection wavelength was set at 238 nm and the flow rate was 0.8 mL/min. Results The common mode of HPLC characteristic chromatographic profile was set up with 19 common peaks, and four of them were identified as oridonin, ponicidin, lasiodonin, and rosmarinic acid. The similarities of 18 batches of RRH were between 0.421—0.984. The result of PCA showed that the accumulative contribution rate of the first three factors was up to 87.3%, which were chosen to comprehensively evaluate the quality of RRH. Conclusion The application of HPLC combined with PCA could quickly and objectively evaluate the quality difference of RRH in different batches.

国家自然科学基金资助项目(81402894)