目的 采用超高效液相色谱-飞行时间质谱(UPLC-Q-TOF-MS^E), 建立南、北五味子化学成分的快速识别模式, 并分析识别南五味子与北五味子化学成分的特征差异性标志物, 为2种五味子的高通量表征与区分提供依据。方法 运用时间依赖型MS^E扫描方式采集样品数据, 根据高分辨质谱数据快速鉴定南、北五味子主要化学成分, 并结合正交偏最小二乘法(OPLS-DA)数据判别模式, 获得2组五味子的特征性差异成分。结果 从南、北五味子色谱图中共鉴定了33个色谱峰, 其中27种为联苯环辛烯类木脂素;经OPLS-DA分析, 正离子模式下, 2种五味子的化学成分差异能得到明显区分, 通过保留时间与质谱信息比对, 识别差异标志物15个, 均为木脂素类成分。结论 为南、北五味子的快速鉴定、质量控制与临床合理应用提供了基础。

Objective To establish a method to fast identify the chemical constituents in the fruits of Schisandra sphenanthera and Schisandra chinensis by ultra performance liquid chromatography tandem with time-of-fight mass spectrometry with MS^E data-acquistion mode (UFLC-Q-TOF-MS^E) and provide the basis for high throughput characterization and distinction of the two two samples of Schisandra Michx by analyzing the remarkably different chemical components in the fruits between S. sphenanthera and S. chinensis. Methods To rapidly identify the main chemical constituents in the fruits between S. sphenanthera and S. chinensis by using time-dependent scan mode, and combine with orthogonal partial least squares discriminant analysis (OPLS-DA) method to acquire significantly different constituents in the two samples of Schisandra Michx. Results A total of 33 compounds were identified including 27 dibenzocyclooctene lignans. The differences of chemical compounds between the two sample groups could be obviously observed through the method of OPLS-DA in positive mode. There were 15 chemical ingredients of lignanes with significant differences identified in the two sample groups by comparison with retention time and mass spectra. Conclusion The present study provides the basis for rapidly qualitative analysis of constituents and quantity control of the fruits in S. sphenanthera and S. chinensis.

国家自然青年科学基金项目(81403106);江西省卫生厅中医药科研计划(2012A027)