[关键词]
[摘要]
目的 研究西藏秦艽Gentiana tibetica花的化学成分。方法 采用正相硅胶、Sephadex LH-20、反相C18柱色谱及制备高效液相等色谱技术进行分离纯化,根据IR、ESI-MS、1H-NMR、13C-NMR分析鉴定化合物结构。结果 从西藏秦艽花的95%乙醇提取物中分离得到12个化合物,分别鉴定为macrophylloside D(1)、orientin 7-caffeate(2)、7-O-feruloylorientin(3)、异牡荆黄素(4)、皂草黄苷(5)、异荭草苷(6)、6'-O-β-D-葡萄糖基龙胆苦苷(7)、獐牙菜苷(8)、獐牙菜苦苷(9)、落干酸(10)、二十一烷醇(11)、蔗糖(12)。结论 化合物1~12均为首次从该植物中分离得到。
[Key word]
[Abstract]
Objective To investigate the chemical constituents in flowers of Gentiana tibetica. Methods The chemical constituents were isolated from the 95% alcohol extract of G. tibetica flowers by silica column chromatography, Sephadex LH-20, C18 and RP-HPLC. Their chemical structures were elucidated on the basis of IR, ESI-MS, 1H-MNR, and 13C-MNR spectroscopic data. Results Twelve compounds, including chromene, flavones C-glycosides, secoiridoid glycosides, iridoid glycosides, aliphatic alcohol, and disaccharide, were obtained from the 95% alcohol extract of G. tibetica flowers, Their structures were identified as macrophylloside D (1), orientin 7-caffeate (2), 7-O-feruloylorientin (3), isovitexin (4), saponarin (5), isoorientin (6), 6'-O-β-D-glucopyranosyl gentiopicroside (7), sweroside (8), swertiamarine (9), loganic acid (10), 1-heneicosanol (11), and sucrose (12). Conclusion Compounds 1—12 are isolated from G. tibetica for the first time.
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[基金项目]
西南民族大学2014级研究生“创新项目”(CX2014SZ49)