目的 研究烟草Nicotiana tabacum叶中的西松烷类化学成分。方法 采用硅胶柱色谱及高效液相色谱等方法进行分离纯化,根据理化性质及波谱数据对化合物的结构进行鉴定。结果 从烟叶95%乙醇提取物中分离得到5个西松烷二萜类成分,分别鉴定为 (1S,2E,4R,6R,7E)-4,6,11-trihydroxy-1-isopropyl-4,8-dimethylpentadeca-2,7-dien-12-one(1)、(1S,2E,4R,6R, 7E,11E)-2,7,11-cembradiene-4,6-diol(2)、(1S,2E,4S,6R,7E,11E)-2,7,11-cembradiene-4,6-diol(3)、(1S,2E,4R,6R,7E,11S)-2,7,12(20)- cembratriene-4,6,11-triol(4)、(1S,2E,4S,6R,7E,11S)-2,7,12(20)-cembratriene-4,6,11-triol(5)。结论 化合物1为新化合物,命名为烟叶二萜A。

Objective To study the cembranoid diterpenes from the leaves of Nicotiana tabacum. Methods The cembranoid diterpenes were isolated and purified by silica gel and preparative HPLC, and their structures were elucidated on the basis of their physicochemistry properties and spectral data. Results Five cembranoid diterpenes were obtained from the ethanol extract of the leaves of N. tabacum and identified as (1S,2E,4R,6R,7E)-4,6,11-trihydroxy-1-isopropyl-4,8-dimethylpentadeca-2,7-dien-12-one (1), (1S,2E,4R,6R,7E,11E)-2,7,11-cembradiene-4,6-diol (2), (1S,2E,4S,6R,7E,11E)-2,7,11-cembradiene-4,6-diol (3), (1S,2E,4R,6R,7E, 11S)-2,7,12 (20)-cembratriene-4,6,11-triol (4), and (1S,2E,4S,6R,7E,11S)-2,7,12(20)-cembratriene-4,6,11-triol (5). Conclusion Compound 1 is a new chain-like cembranoid diterpene named nicotiaditerpene A.

烟草基因组重大专项项目:典型香型烟草品质形成的代谢特征研究(902014AA0530)