[关键词]
[摘要]
目的 建立虎杖药材的HPLC-DAD-ELSD指纹图谱, 对10个不同产地的虎杖药材进行质量评价。方法 使用Luna C18(2)色谱柱(250 mm×4.6 mm, 5 μm), 乙腈-水为流动相进行梯度洗脱, 体积流量1.0 mL/min, 柱温35 ℃, 检测波长254 nm, 进样量10 μL。蒸发光检测器漂移管温度109 ℃, 载气体积流量3.0 L/min。结果 通过10批虎杖药材建立了指纹图谱的共有模式, 其中HPLC-DAD指纹图谱共有峰19个, 相似度为0.938~0.993;HPLC-ELSD指纹图谱共有峰14个, 相似度为0.905~0.999。确认了虎杖苷、白藜芦醇、大黄素-8-O-β-D-葡萄糖苷、大黄素甲醚-8-O-β-D-葡萄糖苷、大黄素和大黄素甲醚。结论 方法准确、稳定, 为客观评价虎杖药材的质量提供了科学依据。
[Key word]
[Abstract]
Objective To establish the chromatography fingerprint of Polygoni Cuspidati Rhizoma et Radix with hyphenated technique of HPLC-DAD-ELSD and to evaluate Polygoni Cuspidati Rhizoma et Radix from 10 different origins. Methods Luna C18 (2) column (250 mm × 4.6 mm, 5 μm) was used. Mobile phase was acetonitrile-H2O; Flow speed was 1.0 mL/min; Temperature of column was set at 35 ℃; Detective wavelength was at 254 nm; Injection volume was 10 μL. The temperature of drift tube was 109 ℃ and the flow speed was 3.0 L/min. Results The chromatography fingerprint of Polygoni Cuspidati Rhizoma et Radix from 10 different origins was established. In the chromatography fingerprint with HPLC-DAD of Polygoni Cuspidati Rhizoma et Radix, 19 common peaks were demarcated and the similarities of Polygoni Cuspidati Rhizoma et Radix were between 0.938—0.993. In the chromatography fingerprint with HPLC-ELSD of Polygoni Cuspidati Rhizoma et Radix, 14 common peaks were demarcated and the similarities of Polygoni Cuspidati Rhizoma et Radix were between 0.905—0.999. Polydatin, resveratrol, emodin-8-O-β-D-glucoside, physcion-8-O-β-D-glucoside, emodin, and physcion were identified. Conclusion The method is accurate and stable, which can be used as the evidence for the quality evaluation of Polygoni Cuspidati Rhizoma et Radix.
[中图分类号]
[基金项目]
吉林省科技厅课题"虎杖有效部位治疗高脂型糖尿病的研究"(201307026YY)