目的 观察山柰酚对2型糖尿病大鼠慢性并发症相关因子的影响。方法 通过高脂饲料喂养, 并ip 30 mg/kg链脲佐菌素(STZ)诱导制备2型糖尿病大鼠模型。山柰酚给药组分别ig给予50、100、200 mg/kg药物, 并设模型组、对照组和二甲双胍(0.2 g/kg)阳性对照组。给药10周后, 采用葡萄糖氧化酶法对各组大鼠血糖进行测定;放射免疫分析法进行血清胰岛素(INS)的检测, 并计算胰岛素敏感指数(ISI);应用酶法对血清中三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、超氧化物歧化酶(SOD)、醛糖还原酶(AR)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的量进行测定;用硫代巴比妥酸(TBA)法检测丙二醛(MDA)的量。结果 与对照组比较, 模型组大鼠血糖及血清INS水平显著升高(P < 0.01), ISI显著降低(P < 0.05);模型组大鼠血清TG和LDL-C水平明显升高(P < 0.01), HDL-C水平显著降低(P < 0.01);模型组大鼠血清MDA、AR、IL-6和TNF-α水平显著升高(P < 0.01), SOD活性显著降低(P < 0.01)。与模型组比较, 山柰酚给药组可降低大鼠血糖水平(P < 0.05、0.01), 改善胰岛素抵抗, 恢复血脂至正常水平, 并伴随着MDA、AR、TNF-α、IL-6水平降低(P < 0.05、0.01), SOD活性升高(P < 0.05、0.01)。结论 山柰酚可能是通过降低血糖、改善ISI, 维持正常血脂水平, 通过抗氧化、抗炎、降低AR途径, 从而对2型糖尿病大鼠的慢性并发症起到保护作用。

Objective To observe the effect of kaempferol on the correlation factors of chronic complications of type 2 diabetic rats. Methods Feeding high-fat diet combined with ip injection of 30 mg/kg STZ in rats to make the model of type 2 diabetes. Rats in every administration group were given respective drug (50, 100, and 200 mg/kg), and set the model, normal control, and positive control (metformin hydrochloride 0.2 g/kg) groups. After 10 weeks, using glucose oxidase to measure blood glucose of rats in each group; Radioimmunoassay was used to measure INS and get ISI. Measuring the contents of TG, HDL-C, LDL-C, SOD, AR, IL-6, TNF-α, and MDA was detected by TBA. Results Compared with diabetic model group, kaempferol administration can reduce blood glucose and insulin resistance, restore normal blood lipid levels, along with reducing MDA, AR, TNF-α, and IL-6 levels and increasing SOD levels. Conclusion Kaempferol can prevent and treat the chronic complications of type 2 diabetic rats by reducing blood glucose, insulin resistance, reducing the AR pathway as well as anti-oxidation and anti-inflammation.