[关键词]
[摘要]
目的 研究方剂配伍对茵陈蒿汤中主要成分提取率的影响。方法 采用HPLC法测定茵陈、栀子、大黄、茵陈与栀子配伍(配伍A)、茵陈与大黄配伍(配伍B)、栀子与大黄配伍(配伍C)及全方配伍(配伍D)中2个环烯醚萜类成分(栀子苷和去乙酰车叶草酸甲酯)、2个西红花酸类成分(西红花苷I和西红花苷II)、3个结合型蒽醌(大黄素-8-O-葡萄糖苷、大黄酚-1-O-葡萄糖苷和大黄素-8-O-葡萄糖苷)、5个游离型蒽醌(芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚)、2个鞣质单体(没食子酸和儿茶素)及绿原酸的提取量, 以单味药中提取率为100%, 计算配伍A~D中15种成分的提取率。结果 配伍A、C、D中栀子苷、西红花苷I和西红花苷II提取率降低;配伍A、D中去乙酰车叶草酸甲酯提取率提高, 配伍C中该成分提取率降低;配伍B中3个结合型蒽醌及芦荟大黄素、大黄酚提取率提高, 配伍C、D中大黄酚和大黄素甲醚提取率提高;配伍C、D中没食子酸提取率降低;配伍A~D中绿原酸提取率降低。结论 方剂配伍对茵陈蒿汤中主要成分提取率有一定影响。
[Key word]
[Abstract]
Objective To research the influence of prescription compatibility on the extraction rate of main constituents in Yinchenhao Decoction (YCHD). Methods The extraction volume of two iridoids (geniposide and deacetylasperulosidic acid methyl ester), two crocetin derivatives (crocin I and crocin II), three bound anthraquinones (aloe-emodin-8-O-glucoside, chrysophanol-1-glucoside, and emodin-8-glucoside), five free anthraquinones (aloe-emodin, rhein, emodin, chrysophanol, and physcion), two tannins (gallic acid and catechin), and chlorogenic acid in Artemisiae Scopariae Herba (ASH), Gardeniae Fructus (GF), Rhei Radix et Rhizoma (RRR), the compatibility of ASH and GF (compatibility A), the compatibility of ASH and RRR (compatibility B), the compatibility of GF and RRR (compatibility C), and the compatibility of ASH, GF, and RRR (compatibility D) were determined by HPLC. Taking the extraction volume in single medicine as 100%, the extraction rates of 15 constituents mentioned above in compatibilities A—D were calculated. Results The extraction rates of geniposide, crocin I, and crocin II in compatibilities A, C, and D, deacetylasperulosidic acid methyl ester in compatibility C, gallic acid in compatibilities C and D, and chlorogenic acid in compatibilities A—D decreased. The extraction rates of deacetylasperulosidic acid methyl ester in compatibilities A and D, three bound anthraquinones, aloe-emodin, and chrysophanol in compatibility B, chrysophanol and physcion in compatibilities C and D increased. Conclusion Prescription compatibility has some influence on the extraction rate of main constituents in YCHD.
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[基金项目]
江苏省中医药局科技项目(HZ07071);江苏省高校自然科学基金项目(08KJB360009)