目的 建立HPLC法同时测定蒙古黄芪药材中的毛蕊异黄酮苷和芒柄花素量的方法, 考察不同产地、加工方法、栽培年限对蒙古黄芪质量的影响。方法 色谱柱为Dikma C₁₈(250 mm×4.6 mm, 5 μm);流动相为乙腈-0.2%甲酸水溶液梯度洗脱;体积流量1.0 mL/min;检测波长260 nm;柱温30 ℃。结果 通过对蒙古地区11个不同产地的40批次蒙古黄芪药材进行分析, 比较毛蕊异黄酮苷和芒柄花素量得出, 蒙古黄芪的产地加工方式最佳为清洗后自然晾晒干燥;巴彦淖尔市乌拉特中旗明安镇头份子地区为蒙古黄芪最佳栽培种植基地;生长年限二年、三年的蒙古黄芪栽培品种质量差异不大;蒙古黄芪野生品种质量优于栽培品种。结论 该方法简单快捷, 适合于黄芪中异黄酮类化合物的定量测定研究, 为今后蒙古黄芪药材的栽培种植和加工方式合理标准的制定提供科学、可靠的依据。

Objective An HPLC method was established to determine the content of calycosin-7-O-β-D-glucoside and formononetin in Astraglus membranaceus var. mongholicus. To study the influence of habitats, processing methods, and planting ages on the quality of A. membranaceus var. mongholicus. Methods The HPLC condition was as follows: column: Dikma C₁₈ (250 mm × 4.6 mm, 5 μm); mobile phase: Acetonitrile-0.2% formic acid (gradient elution); flow speed: 1.0 mL/min; detection wavelength: 260 nm; temperature of column: 30 ℃. Results Comparing the content of calycosin-7-O-β-D-glucoside and formononetin in 40 samples of A. membranaceus var. mongholicus from 11 different habitats, we could get the following: The processing methods of A. membranaceus var. mongholicus were cleaning and natural drying; Bayinnaoer city Wu Qianqi ming'an town head part was the best habitat for A. membranaceus var. mongholicus. The quality of A. membranaceus var. mongholicus cultivars grown for two years and three years had no difference. The quality of wild species was superior cultivars. Conclusion The method is simple and rapid for determination, the contents of calycosin-7-O-β-D-glucoside and formononetin in A. membranaceus var. mongholicus. It can be used as a scientific and reliable basis for formulating the reasonable standard of cultivating and processing methods of A. membranaceus var. mongholicus.

国家科技支撑项目子课题——蒙古黄芪特色产品的综合开发、药材及饮片商业标准研究(2011BAI07B01)