目的 研究杜仲Eucommia ulmoides不同粒径粉体理化特性及体外溶出行为的差异, 为杜仲超微粉体的粒径控制与应用提供实验依据。方法 采用超微粉碎机制备杜仲不同粒径的超微粉体, 激光粒度仪测定粉体的粒径, 显微镜观察显微结构, 通过破壁率来衡量超微粉碎的破壁效果;利用HPLC法测定不同粒径粉体有效成分提取效率及在不同溶出介质的溶出度。结果 杜仲超微粉体在显微结构、破壁率、休止角及堆密度等方面与普通粉体差异显著;超微粉体的提取率以及在水、人工胃液、人工肠液中有效成分的溶出度均高于普通粉体。结论 适度的微粉化能提高杜仲细胞破壁率, 促进杜仲有效成分的溶出, 其溶出度还受到溶出介质的影响, 超微粉碎技术应用于杜仲具有可行性。

Objective Comparing the differences about physicochemical properties and in vitro dissolution behavior of active ingredients between ultrafine powder and common powder of Eucommia ulmoides to provide the experimental evidence for the ultrafine powder application and the control of particle size. Methods Ultrafine powders were produced by using ultra mill. Particle size, cell wall breaking rate, morphology, and dissolution were used to evaluate the effect of particle size on microcharacteristic and dissolution behavior of powders of E. ulmoides. Results There were significant differences between ultrafine and common powders of microcharacteristic. The extraction rates and dissolution rates of the active ingredients of ultrafine powders were higher than those of common powders. Conclusion An appropriate degree of superfine grinding can increase the wall-breaking rate and promote the dissolution of active ingredients of E. ulmoides, and the dissolution medium has an obvious influence on the dissolution rates. The application of ultrafine grinding technology to E. ulmoides is feasible.

国家科技支撑计划资助课题(2012BAC11B02)