目的 建立RP-HPLC同时测定舒胸方中盐酸川芎嗪、阿魏酸、羟基红花黄色素A、人参皂苷Rg₁、人参皂苷Rb₁、人参皂苷Re、三七皂苷R₁的方法。方法 以舒胸方为研究对象, 采用RP-HPLC法, 以Inertsil ODS-SP(250 mm×4.6 mm, 5 μm)为分析柱, 乙腈-0.05%磷酸水溶液为流动相, 梯度洗脱(0～45 min, 15%乙腈;45～60 min, 15%～20%乙腈;60～80 min, 20%～38%乙腈;80～90 min, 15%乙腈), 检测波长为203 nm, 柱温30 ℃, 体积流量1.0 mL/min, 进样量为20 μL。结果 7种成分盐酸川芎嗪、阿魏酸、羟基红花黄色素A、人参皂苷Rg₁、人参皂苷Rb₁、人参皂苷Re、三七皂苷R₁线性范围分别为0.101 0～1.364、0.064 0～0.867 0、1.020～13.77、1.699～22.95、1.869～25.23、0.171 4～2.314、0.516 0～6.966 μg/mL, 线性关系良好, r值均在0.999 3～0.999 9, 平均加样回收率在97.25%～103.52%, RSD均<3%。结论 该方法简便、准确可靠, 重复性好, 分离度好, 适用于同时测定舒胸方中主要7种有效成分。

Objective To establish a RP-HPLC method for the determination of ligustrazine hydrochloride, ferulic acid, hydrosafflower yellow A, ginsenoside Rg₁, ginsenoside Rb₁, ginsenoside Re, and notoginsenoside R₁ in Shuxiong prescription. Methods The Inertsil ODS-SP (250 mm × 4.6 mm, 5 μm) was used. The mobile phase, consisting of acetonitrile-0.05% phosphoric acid solution, was programmed for a gradient elution. The flow rate was 1.0 mL/min, the detection wavelength was 203 nm, and the column temperature was 30 ℃. Results The excellent linearity with correlation coefficients (r) of 0.999 3—0.999 9 was obtained. The average recoveries of the seven compounds were 97.25%—103.52% and all RSD values were less than 3%. Conclusion The method appears to be simple, accurate, and well reproducible, which could be used for the simultaneous determination of the above-mentioned seven compounds in Shuxiong prescription.

重庆市科委自然科学基金项目(CSTC2012jjA10012)