目的 建立枳实Aurantii Immaturus Fructus中4种黄酮类成分芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷的一测多评法,验证该方法在枳实质量评价中应用的准确性及可行性。方法 以橙皮苷为内标,分别建立橙皮苷与芸香柚皮苷、柚皮苷、新橙皮苷的相对较正因子,计算枳实中芸香柚皮苷、柚皮苷、新橙皮苷的量,实现一测多评。同时采用外标法测定枳实中4种黄酮类成分的量,并比较计算值与实测值的差异,以验证一测多评法在测定中的科学性及可行性。结果 各相对校正因子重复性良好,一测多评法测定结果与外标法测定结果无显著差异。结论 以橙皮苷为内标同时测定芸香柚皮苷、柚皮苷、新橙皮苷的一测多评法可用于枳实的定量分析。

Objective To establish a method for simultaneously determination for the content of four flavones (narirutin, naringin, hesperidin, and neohespridin) in Aurantii Immaturus Fructus by single marker, which is feasible and accurate. Methods Taking hesperidin as internal standard substance, to establish a method for quantitative analysis of multi-components with single marker. To determinate the content of narirutin, naringin, hesperidin, and neohespridin in Aurantii Immaturus Fructus. The contents in 10 batches of samples were determined by both external standard method and QAMS. The The scientificity and feasibility of the method were evaluated by comparison of the quantitative results between external standard method and QAMS. Results The relative correction factor (RCF) was perfect. The results calculated with a single marker were consistent with the results by the external standard method. Conclusion The method with a single marker is accurate and feasible to evaluate the quality of Aurantii Immaturus Fructus.

国家"十二五"科技支撑计划项目(201113AI04B06)