目的 研究益母草Leonurus japonicus的低极性化学成分。方法 采用硅胶、Sephadex LH-20以及反相C₁₈柱色谱等方法进行分离纯化,根据理化性质及波谱数据鉴定化合物结构。结果 从益母草的醋酸乙酯提取部位分离得到6个化合物,包括叠烯类化合物2个:(-)-十九碳-5,6-二烯酸甲酯(1)、十八碳-5,6-二烯酸甲酯(2);饱和脂肪族化合物4个:二十一烷酸(3)、花生酸(4)、二十七烷酸(5)和肉豆蔻酸甲酯(6)。结论 所有化合物均为首次从益母草中分离得到,其中化合物1为新化合物,命名为益母草叠烯酸酯A,化合物2为首次从益母草属植物中分离得到。

Objective To study the low polar chemical constituents from Leonurus japonicus. Methods The chemical constituents were isolated and purified by various chromatographic methods including column chromatography over silica, Sephadex LH-20, and Rp C₁₈ columns. Their structures were identified by physicochemical properties and spectroscopic analysis. Results Six aliphatic compounds were isolated from the EtOAc extracts of L. japonicus, including two allenes: (-)-nonadeca-5,6-dienoic acid methyl ester (1) and methyl octadeca-5,6-dienoate (2); four saturated aliphatic compounds: heneicosanoic acid (3), arachidic acid (4), heptacosanoic acid (5), and methyl myristate (6). Conclusion All the compounds are isolated from L. japonicus for the first time. Compound 1 is a new one named leonuallenote A, while compound 2 is first reported from the plants of Leonurus L.

国家自然科学基金资助项目(81303209);中国博士后科学基金项目(2014M562291);四川省应用基础研究项目(2012JYZ005);四川省科技支撑计划(2011SZ0056)