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[摘要]
目的 建立新疆不同产地雪菊的UPLC指纹图谱,为雪菊的真伪鉴别和质量控制提供依据.方法 采用Waters ACQUITY UPLC HSS T3 C18(150 mm×2.1 mm,1.8 μm)色谱柱,以乙腈-pH 3.0磷酸水溶液为流动相,体积流量0.2 mL/min,检测波长280 nm,柱温30 ℃,以儿茶素、木犀草苷、3,5-O-二咖啡奎宁酸为参照物,对13批次新疆不同产地雪菊进行指纹图谱分析.结果 建立了新疆产雪菊的UPLC指纹图谱,标定共有峰17个,并用对照品指认了3个色谱峰,测定了13批次新疆产雪菊中3种成分的量.结论 13批次新疆产雪菊相似度虽然存在微小差异,但均大于0.90.根据该色谱条件构建的UPLC指纹图谱为新疆产雪菊质量评价提供了科学依据.
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[Abstract]
Objective In order to identify the authenticity and control the quality of Xinjiang chrysanthemum, the fingerprinting of it was established using UPLC and discussed in this paper. Methods Thirteen batches of Xinjiang chrysanthemum from different origins were analyzed by UPLC with catechin, luteoloside, quercetin, and 3,5-di-O-caffeoyl quinic acid as reference substances. The chromatographic condition was performed on Waters ACQUITY UPLC HSS T3 C18 (150 mm × 2.1 mm, 1.8 μm) and eluted with mobile phase containing acetonitrile-pH 3.0 phosphoric acid solution. The flow rate was 0.2 mL/min with detection wavelength 280 nm in the temperature of 30 ℃. Results There were 17 peaks in the UPLC fingerprint of Xinjiang chrysanthemum and three components were identified by three peaks determined using reference substance. Conclusion The similarity of 13 batches is little difference, but is more than 0.90. The results showed that the UPLC fingerprint chromatogram could be used as a valuable method for the quality evaluation of Xinjiang chrysanthemum.
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