[关键词]
[摘要]
目的 利用响应面法优选气血双补酒的渗漉提取工艺.方法 采用单因素试验结合Box-Behnken中心组合设计方法,对提取工艺中乙醇体积分数、溶剂倍量、渗漉速率3个因素进行考察,以复方中6个指标性成分(人参皂苷Rb1、毛蕊异黄酮葡萄糖苷、阿魏酸、二苯乙烯苷、芍药苷、橙皮苷)及浸出物的质量浓度的综合评分作为考察指标进行提取工艺优化研究.结果 最佳提取工艺为以10倍量48%乙醇,1.4 mL/min的体积流量渗漉提取.结论 响应面法优选气血双补酒提取工艺简便、合理可行,具有较高的精确度,可为工业化大生产提供科学的依据.
[Key word]
[Abstract]
Objective To optimize the percolation extraction technology for Qixue Shuangbu Tinctura by response surface methodology (RSM). Methods The single factor experiment combined with Box-Behnken design was used to optimize the extracting technology, with six major characteristic components (ginsenoside Rb1, calycosin 7-O-β-D-glucopyranoside, ferulic acid, stilbene glucoside, paeoniflorin, hesperidin) and extract of Qixue Shuangbu Tinctura as indexes, in order to detect three factors, including the alcohol volume fraction, the dosage of ethanol, and the percolate speed, and optimize the extraction process of Qixue Shuangbu Tinctura. Results Optimum percolation process was added 10 times of the amount of 48% ethanol with percolation speed of 1.4 mL/min. Conclusion This optimized extraction technology of Qixue Shuangbu Tinctura is reasonable, stable, and feasible, and with high accuracy, which could be extended to the applications of large-scale production.
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[基金项目]
国家中医药管理局公益性行业专项(2015468002-3);江苏省科技支撑计划——工业项目(BE2012011)