目的 建立益智仁Alpiniae Oxyphyllae Fructus的超高效液相(UPLC)指纹图谱。方法 采用ACQUITY UPLC HSS T3色谱柱(100 mm×2.1 mm,1.8 μm);以乙腈-甲酸水溶液为流动相梯度洗脱;体积流量0.5 mL/min;样品室温度10 ℃;柱温30 ℃;检测波长254 nm;进样量2 μL。结果 首次建立了益智仁的UPLC特征指纹图谱共有模式,标定了20个共有峰,结合保留时间和紫外光谱分析,确定了原儿茶酸、α-羟甲基糠醛、oxyphyllenodiol A、teuhetenone A、teuhetenone B、白杨素、山柰素、杨芽黄素、圆柚酮共9个化合物。19批益智仁,其中15批的相似度在0.970以上。结论 该方法快速、高效,可用于益智仁的质量评价。

Objective To establish the fingerprint of Alpiniae Oxyphyllae Fructus by UPLC. Methods The chromatographic fingerprint was obtained with ACQUITY UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm) and gradient eluted with acetonitrile and aqueous formic acid. The flow rate was 0.5 mL/min, the sample room temperature and the column temperature were maintained at 10 ℃ and 30 ℃, respectively. The detection wavelength was set at 254 nm. The injection volume was 2 μL. Results The common mode of UPLC fingerprint of Alpiniae Oxyphyllae Fructus was set up firstly. There were 20 common peaks in the fingerprints. Protocatechuic acid, α-hydroxymethyl furfural, oxyphyllenodiol A, teuhetenone A, teuhetenone A, chrysin, kaempferide, tectochrysin, and nootkatone were identified by comparing the retention time and their ultraviolet spectra. The similarities of 15 Alpiniae Oxyphyllae Fructus among 19 batches of Alpiniae Oxyphyllae Fructus were above 0.970. Conclusion The method is rapid and efficiency. It can be used for the quality evaluation of Alpiniae Oxyphyllae Fructus.

中药药效物质基础筛选与评价重点实验室(F13-287-1-00);辽宁省自然科学基金项目(2011412004-1)