[关键词]
[摘要]
目的 分析丹参柯巴基焦磷酸合酶(CPS)的氨基酸序列特征,并与其他植物进行比较,为丹参CPS蛋白的后续研究提供有利参考。方法 利用生物信息学方法,以丹参为主,对13科16种植物的18条CPS蛋白序列进行了序列组成、生化特性、导肽、信号肽、跨膜结构域、疏水性/亲水性、蛋白质二级、三级结构及功能域等预测分析,并进行了丹参CPS蛋白序列与其他植物的同源比对以及系统进化树构建。结果 CPS蛋白氨基酸残基数集中在730以上;相对分子质量91 610左右;平均理论等电点5.87,提示CPS为酸性蛋白。序列结构预测结果显示,CPS蛋白具有明显的疏水区和亲水区,不存在信号肽,不具有跨膜结构域,可能有叶绿体转运肽。蛋白质二级结构中最主要的结构元件是α-螺旋和无规则卷曲。蛋白同源性比对结果显示,丹参CPS与同科植物迷迭香和玄参科植物野甘草的同源性最高。结论 对丹参等植物的CPS蛋白进行了详细的生物信息学分析,可为今后深入研究该酶的结构特征和功能提供参考。
[Key word]
[Abstract]
Objective To provide the useful information for the further study of copalyl diphosphate synthase (CPS) of Salviae Miltiorrhizae Radix et Rhizoma through amino acid (aa) sequences comparison in S. miltiorrhiza and other plants. Methods Bioinformatics analysis methods were used to perform the prediction of composition and physicochemical character, leader peptide, signal peptide, transmembrane domain, hydrophobicity/hydrophilicity, secondary structure, tertiary structure, and functional domain of 18 CPS amino acid sequences in S. miltiorrhiza and other different plants which belong to 16 species and 13 families. Homology analysis and phylogenetic trees of CPS were also carried out. Results Most CPS proteins contain more than 730 aa. Their molecular weight is approximately 91 610 and the isoelectric point of them is about 5.87, which suggests that CPS is acidic protein. The results of the sequence structure prediction show that CPS has apparent hydrophobic region and hydrophilicity region, no signal peptide, no transmembrane domain, and may probably have chloroplast transit peptide. The main secondary structures of the proteins are α-helix and random coil. Homology analysis reveals that S. miltiorrhiza has the highest homology comparied with Rosmarinus officinalis and Scoparia dulcis. Conclusion This work provides a systemic sequence analysis of CPS in S.miltiorrhiza and other plants. It will provide the useful information for CPS studies in the future.
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[基金项目]
国家自然科学基金资助项目(81325024,81303160)