目的 建立利用分子印迹技术从桂枝茯苓胶囊提取物中定向分离制备去氢土莫酸的方法。方法 以去氢土莫酸为分子模板,采用溶胶-凝胶法制备了去氢土莫酸分子印迹聚合物,并对其吸附性能进行研究。以此聚合物为填料,从桂枝茯苓胶囊提取物中一步分离制备得到去氢土莫酸,根据理化性质和波谱数据鉴定其结构。结果 经Scatchard分析,去氢土莫酸分子印迹聚合物最大表观结合位点数(Q_max)为9.10 mg/g。经HPLC检测去氢土莫酸质量分数为90.76%。结论 该方法可用于从桂枝茯苓胶囊提取物中靶向分离制备去氢土莫酸,有利于减少提取过程中有机溶剂的使用,操作简单,为其高效分离纯化提供新的方法。

Objective To establish a method for directional separation of dehydrotumulosic acid from the extracts of Guizhi Fuling Capsule with molecular imprinting technique (MIT). Methods Molecular imprinting polymer (MIP) was prepared by sol-gel process with dehydrotumulosic acid as molecular template to study the absorption property. The dehydrotumulosic acid was achieved from Guizhi Fuling Capsule by one-step separation with polymer as filler. The structure of dehydrotumulosic acid was identified on the basis of the spectral data and physicochemical property. Results The maximum binding capacity (Q_max) of MIP was 9.10 mg/g measured by Scatchard equation and the purity of dehydrotumulosic acid was 90.76% by HPLC. Conclusion The established MIT for the directional separation of dehydrotumulosic acid from Guizhi Fuling Capsule is simple and benefit to reducing the solvent use during the separation process, which could offer a novel method for the separation and purification of dehydrotumulosic acid.

科技部重大新药创制:现代中药创新集群与数字制药技术平台(2013ZX09402203)