目的 研究香青兰有效成分的提取工艺并比较不同产地香青兰中有效成分的量。方法 采用HPLC法测定香青兰中木犀草素-7-O-葡萄糖醛酸苷(I)、芹菜素-7-O-葡萄糖醛酸苷(II)、迷迭香酸、香叶木素-7-O-葡萄糖醛酸苷(III)、田蓟苷和刺槐素-7-O-葡萄糖醛酸苷(IV)的量。在单因素试验的基础上,采用正交试验考察提取溶媒、乙醇用量和提取时间对提取工艺的影响,确定最佳提取工艺。根据最佳提取工艺,比较不同产地香青兰中I、II、迷迭香酸、III、田蓟苷和IV的量。结果 最佳提取条件为加30倍量的40%乙醇水溶液,提取1次,5 h。新疆吉木萨尔产的香青兰中I、II、迷迭香酸、III、田蓟苷和IV的量较高。结论 该提取工艺合理、稳定、可行;不同产地的香青兰药材中I、II、迷迭香酸、III、田蓟苷和IV的量存在一定的差异。

Objective To study the extraction technology for the active constituents in Dracocephali Moldavici Herba (the aerial parts of Dracocephalum moldevica) and to compare their contents Dracocephali Moldavici Herba from various habitats. Methods The contents of luteolin-7-O-glucuronide (I), apigenin-7-O-glucuronide (II), rosmarinic acid, diosmetin-7-O-glucuronide (III), tilianin, and acacetin-7-O-glucuronide (IV) in Dracocephali Moldavici Herba were measured using HPLC method. Based on single-factor test, and the influence of the extracting menstrua, menstruum dosage, and extracting time were investigated using orthogonal design method. Based on the optimal technology, the contents of I, II, rosmarinic acid, III, tilianin, and IV in Dracocephali Moldavici Herba from various habitats were compared. Results The optimal conditions for the extracting for one time, each time for 5 h with 50 times of amount of 40% ethanol. D. moldevica produced in Jimusar, Xinjiang had the higher contents of I, II, rosmarinic acid, III, tilianin, and acacetin-7-O- glucuronide. Conclusion This extraction technology is reasonable, stable, and feasible. The contents of I, II, rosmarinic acid, III, tilianin, and IV in Dracocephali Moldavici Herba from different habitats have some differences. The multi-index contents of I, apigenin-7-O-glucuronide, rosmarinic acid, III, tilianin, and IV could reflect the quality of Dracocephali Moldavici Herba more comprehensively.

国家科技重大专项"重大新药创制"课题(2012ZX09102201-009)