目的 对桂枝茯苓胶囊内容物的正丁醇和醋酸乙酯萃取部位进行化学成分研究。方法 采用硅胶柱色谱、葡聚糖凝胶LH-20及HPLC等色谱技术进行分离纯化,并利用NMR等波谱学手段对化合物进行结构鉴定。结果 从醋酸乙酯层分离得到7个单体化合物,分别鉴定为苯甲酰芍药苷(1)、白芍苷R₁(2)、paeonidanin A(3)、4-甲氧基苯甲酰基芍药苷(4)、paeonidanin B(5)、4-O-methylgalloylpaeoniflorin(6)、paeoniflorin B(7);从正丁醇层中分离得到4个化合物,分别鉴定为isomaltopaeoniflorin(8)、芍药苷(9)、羟基芍药苷(10)、芍药内酯苷(11)。结论 化合物1～8为首次从该复方中分离得到,化合物1～11均为芍药苷类化合物。

Objective To investigate the chemical constituents from the ethyl acetate and n-butanol extact of Guizhi Fuling Capsule. Methods The compounds were isolated by chromatography on silica gel, Sephdex LH-20 columns, and prep-HPLC. The chemical structures were identified by NMR methods, respectively. Results Eleven compounds were isolated from the ethyl acetate extact. They were identified as benzoylpaeoniflorin (1), albiflorin R₁ (2), paeonidanin A (3), 4-methylbenzoylpaeoniflorin (4), paeonidanin B (5), 4-O-methylgalloylpaeoniflorin (6), and paeoniflorin B (7). Four compounds were isolated from the n-butanol extact and were identified as isomaltopaeoniflorin (8), paeoniflorin (9), oxypaeoniflorin (10), and albiflorin (11). Conclusion Compounds 1—8 are isolated from Guizhi Fuling Capsula for the first time, and compounds 1—11 belong to the category of peaoniflorin.

科技部重大新药创制:现代中药创新集群与数字制药技术平台(2013ZX09402203)