通过查阅PubMed、中国知网、万方等数据库搜集相关文献,同时采用生物反应路径分析(ingenuity pathway analysis, IPA)系统推测丹蒌片与相关疾病的作用靶点,预测相关的作用机制。近几年对丹蒌片的研究主要集中在高脂血症及动脉粥样硬化等疾病方面,一些已知的化学成分可能是通过环加氧酶2(PTGS2)、瘦素蛋白(LEP)、一氧化氮合酶3(NOS3)和低密度脂蛋白受体(LDLR)起作用。明确丹蒌片化学成分及其药理作用还具有一定的困难,生物化学与网络药理学的结合有助于了解丹蒌片对心血管疾病治疗的作用机制。

By systematically searching through the literature databases, such as PubMed, China National Knowledge Infrastructure (CNKI), and WanFang database and applying Ingenuity Pathway Analysis (IPA) software, we have identified the potential disease targets and forecasted the possible mechanisms of several chemical components in Danlou Tablets. We found that the pharmacological study on Danlou Tablets in recent years mainly focused on hyperlipidemia and atherosclerosis. And some of the identified chemical components have been shown to exert such effects through the cyclooxygenase 2 (PTGS2), leptin (LEP), nitric oxide synthase (NOS3), and low density protein receptor (LDLR). However, it remains a great challenge in identifying the key active chemical components of Danlou Tablets and elucidating their pharmacological effects. The combination of the chemical biology and network pharmacology approaches will facilitate our understanding on the cardiovascular disease specificity and mechanisms of the action for Danlou Tablets.

国家重点基础研究发展计划("973"计划,2014CB542902);国家自然科学基金资助项目(81274128,81303183)