双水相萃取是一种易于放大、可连续化操作、易于集成技术、绿色环保的新型液/液萃取分离技术,在分离纯化蛋白质、基因、生物纳米分子、细胞和天然产物等领域有着广泛应用。系统综述近5年该技术在中药活性成分提取分离中的应用进展,以期抛砖引玉,推动中药活性成分分离纯化研究的进步和发展。

Aqueous two-phase system (ATPS) is a new liquid-liquid fractionation technique that has been extensively applied in the separation and purification of natural products including proteins, genetic material, bionanoparticles, cells, and secondary metabolites. Advantages of the technique include scale-up potential, continuous operation, ease of process integration, low toxicity, etc. This paper reviewed the applications in separation and purification of the active constituents from Chinese materia medica (CMM) in recent five years, so as to promote the development of separation of the active constituents in CMM.

湖南省自然科学基金资助项目(14JJ6037);张家界科技局植物开发基础研究专项(2013YB26);吉首大学2013年人才引进专项(jsdxrcyjkyxm201301)