[关键词]
[摘要]
目的 研究盆架树Winchia calophylla树皮的生物碱成分。方法 采用硅胶和Sephadex LH-20 柱色谱等方法进行分离和纯化,通过MS、NMR等波谱数据以及X-射线单晶衍射分析进行结构鉴定,采用MTT法测试化合物对7种人体肿瘤细胞(肺癌A549,乳腺癌MCF-7,前列腺癌PC-3,神经胶质瘤U87MG,多发性骨髓瘤U266、MM1.S、MM1.R)增殖的抑制作用。结果 从盆架树树皮乙醇提取物的总生物碱部分分离得到1个新单萜吲哚生物碱,命名为盆架树碱B(1)。结论 化合物1为新化合物,该化合物对7种人体肿瘤细胞株无增殖抑制作用。
[Key word]
[Abstract]
Objective To investigate the alkaloid constituents from the trunk barks of Winchia calophylla. Methods The constituents were isolated and purified by column chromatography over silica gel and Sephadex LH-20 columns. The structure of the isolated compound was elucidated by MS and NMR spectra, and then confirmed by a single-crystal X-ray crystallographic analysis. The isolated compound was also evaluated for its in vitro cytotoxicity against the proliferation of seven kinds of human cancer cell lines (lung cancer: A549, breast cancer: MCF-7, prostate cancer: PC-3, glioma: U87MG, multiple myeloma: U266, MM1.S, and MM1.R). Results A new monoterpenoid indole alkaloid, identified as calophyline B (1), was isolated from the ethanol extract of the trunk barks of W. calophylla. Conclusion Compound 1 is a new compound, which shows no cytotoxicity against the proliferation of the above human tumor cells.
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[基金项目]
国家自然科学基金资助项目(21172024);教育部科学技术研究重点项目(212151);四川省科技计划项目青年基金(2014JQ0049)