目的 采用RP-HPLC法测定杜仲板皮和枝皮中桃叶珊瑚苷、绿原酸、京尼平苷和松脂醇二葡萄糖苷的量.方法 采用Cosmosil C₁₈(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-0.1%磷酸水溶液进行梯度洗脱,体积流量0.8 mL/min,检测波长208 nm,柱温25 ℃.结果 杜仲板皮中桃叶珊瑚苷、松脂醇二葡萄糖苷量大于枝皮,枝皮绿原酸、京尼平苷量大于板皮;在不同产地来源上,杜仲板皮和枝皮中桃叶珊瑚苷、绿原酸、京尼平苷和松脂醇二葡萄糖苷平均质量分数有一定差异性.结论 该方法简便、快速、准确可靠,为更全面合理科学地对杜仲药材质量的评价提供了技术支撑.

Objective To determine the contents of aucubin, chlorogenic acid, geniposide, and pinoresinol diglucoside in the slab and branch barks of Eucommia ulmoides. Methods The separation was performed on a Cosmosil C₁₈ (250 mm ×4.6 mm, 5 μm) column with the gradient elution acetonitrile-0.1% phosphoric acid; The flow rate was 0.8 mL/min; The detection wavelength was 208 nm; The column temperature was at 25 ℃. Results The average content of aucubin: slab bark > branch bark; The average content of chlorogenic acid: branch bark > slab bark; The average content of geniposide: branch bark > slab bark; The average content of pinoresinol diglucosid: slab bark > branch bark. In different origins, the average contents of the above four constituents are more certain differences. Conclusion The method is simple, rapid, accurate, and there are significant differences in the contents of aucubin, chlorogenic acid, geniposide, and pinoresinol diglucosid from the different parts of E. ulmoides, which would provide the better technique support for the quality control of E. ulmoides.

江西省青年科学家培养对象项目(20142BCB23022);江西创新药物与高效节能降耗制药设备协同创新中心项目(赣教高字[2012]89号)