目的 观察姜黄素对5种非白念珠菌菌丝及生物膜形成的抑制作用.方法 测定姜黄素对热带念珠菌Candida tropicalis、光滑念珠菌C. glabrata、近平滑念珠菌C. parapsilokis、克柔念珠菌C. Krusei、季也蒙念珠菌C. guilliermondii的最小抑菌浓度(MIC)以及抑制50%生物膜浓度(SMIC₅₀),利用光学显微镜观察姜黄素对非白念珠菌菌落形态的影响,利用倒置显微镜与扫描电镜分别观察姜黄素对非白念珠菌菌丝形成以及生物膜形成的影响.结果 姜黄素对热带念珠菌、光滑念珠菌、近平滑念珠菌、克柔念珠菌、季也蒙念珠菌的MIC分别为64、128、256、256、128 μg/mL;SMIC₅₀分别为512、512、>512、>512、512 μg/mL;倒置显微镜与扫描电镜观察发现姜黄素对5种非白念珠菌菌丝以及生物膜的形成均有抑制作用.光学显微镜观察发现姜黄素能抑制这5种非白念株菌在固体培养基上菌落周边菌丝的形成.结论 姜黄素对5种非白念珠菌菌丝及生物膜的形成具有抑制作用.

Objective To observe the inhibitory effects of curcumin on hyphal development and biofilm formation of five kinds of non-Candida albicans. Methods Serial 2-fold dilution assay was used to determine the MICs of curcumin to C. tropicalis, C. glabrata, C. parapsilokis, C. krusei, C. guilliermondii; XTT assay was used to determine the SMIC₅₀ of curcumin to the five non-C. albicans. Inverted microscope and scanning electron microscope (SEM) were applied to inspectting the morphological change of non-C. albicans treated by curcumin, The dilution method was applied to inspecting the hyphae around the colonies. Results The MICs of curcumin to C. tropicalis, C. glabrata, C. parapsilokis, C. Krusei, C. guilliermondii were 64, 128, 256, 256, and 128 μg/mL, and the SMIC₅₀ were 512, 512, >512, >512, and 512 μg/mL, respectively. We found that curcumin could inhibit the hyphal and biofilm formation of the five kinds of non-C. albicans by observation with inverted microscope and SEM, and curcumin could inhibit the hyphal development around the colonies by observation with optical microscope. Conclusion Curcumin could inhibit the hyphal development and biofilm formation of the five kinds of non-C. albicans.

国家自然科学基金资助项目(81073127);安徽省自然科学基金项目(1408085MH165);安徽中医药大学校级科研项目(2014zr026)