目的 研究青龙衣(核桃楸Juglans mandshurica的未成熟的外果皮)正丁醇部位的化学成分.方法 采用正、反相硅胶柱色谱,Sephadex LH-20,高效液相制备色谱等方法进行分离纯化,并通过波谱数据分析鉴定化合物的结构.结果 从青龙衣正丁醇提取部位中分离得到14个化合物,分别鉴定为5-O-咖啡酰基奎宁酸丁酯(1)、3,5-二咖啡酰奎宁酸丁酯(2)、香草酸-4-O-β-D-(6'-O-没食子酰基) 葡萄糖苷(3)、4-羟基-2,6-二甲氧基苯酚-1-O-β-D-吡喃葡萄糖苷(4)、4,5,8-三羟基-α-萘酮-5-O-β-D-吡喃葡萄糖苷(5)、1,4,8-三羟基萘-1-O-β-D-吡喃葡萄糖苷(6)、1,4,5-三羟基萘-1,4-二-O-β-D-吡喃葡萄糖苷(7)、山柰酚-3-O-β-D-吡喃葡萄糖苷(8)、槲皮素-3-O-β-D-吡喃葡萄糖苷(9)、槲皮苷(10)、杨梅苷(11)、阿福豆苷(12)、金丝桃苷(13)、胡萝卜苷(14).结论 化合物1～4为首次从胡桃属植物中分离得到,化合物5、8～12为首次从青龙衣中分离得到.

Objective To study the chemical constituents in n-butanol extracts from the epicarp of green fruits of Juglans mandshurica. Methods The compounds were isolated and purified by silica gel, ODS, Sephadex LH-20 columns, high preparative performance liquid chromatography, etc. And their structures were identified by spectral analysis. Results Fourteen compounds were obtained and identified as 5-O-cafffeoyl quinic acid butyl ester (1), 3,5-di-O-caffeoylquinic acid butyl ester (2), vanillic acid-4-O-β-D-(6'-O-galloyl) glucopyranoside (3), 4-hydroxy-2,6-dimethoxyphenol-1-O-β-D-glucopyranoside (4), 4,5,8-trihydroxy-α-tetralone-5-O-β-D-glucopyranoside (5), 1,4,8-trihydroxynaphthalene-1-O-β-D-glucopyranoside (6), 1,4,5-trihydroxynaphthalene-1,4-di-O-β-D-glucopyranoside (7), kaempferol-3-O-β-D-glucopyranoside (8), quercetin-3-O-β-D-glucopyranoside (9), quercitrin (10), myricitrin (11), afzelin (12), hyperin (13), and daucosterol (14). Conclusion Compounds 1-4 are isolated from the plants of Juglans L. for the first time, Compounds 5, 8-12 are isolated from the epicarps of J. mandshurica for the first time.

国家自然科学基金资助项目(81202890);中国博士后特别资助(2014T70374);中国博士后面上资助(一等)(2013M530164);黑龙江省博士后基金(LBH-Z13195);黑龙江省教育厅科学技术研究项目(12541818);哈尔滨市青年科技创新人才项目(2013RFQXJ052);黑龙江中医药大学博士创新基金项目(B201103);黑龙江中医药大学优秀创新人才支持项目(051248)