[关键词]
[摘要]
目的 利用HPLC法对翠菊不同部位中的5种黄酮类成分同步检测.方法 色谱柱为XUnion C18(250 mm×4.6 mm,5 μm);流动相为乙腈(A)-水(B),梯度洗脱,0~65 min,13%~45%A;体积流量 1 mL/min;柱温25 ℃;检测波长210 nm;进样量20 μL,测定翠菊花瓣、花托、花杆75%乙醇提取物,醋酸乙酯萃取部位中芹菜素、大波斯菊苷、山柰酚、金丝桃苷、木犀草素的量.结果 金丝桃苷、大波斯菊苷、木犀草素、芹菜素、山柰酚色谱分离良好,均在2.5~200 mg/L线性关系良好,r均大于0.999 0,加样回收率为95%~105%.结论 方法快速、准确,可为翠菊不同部位的质量控制提供依据.
[Key word]
[Abstract]
Objective To establish an HPLC method for the simultaneous determination of five flavonnid components in different parts of Callistephus chinensis. Methods The chromatographic separation was performed on an XUnion C18 column (250 mm × 4.6 mm, 5 μm) with a gradient of acetonitrile and water, at a flow rate of 1 mL/min, and detected at 210 nm. Results The calibration curve was linear within 2.5—200 mg/L for hyperoside, apigenin-7-O-β-D-glucopyranoside, luteolin, apigenin, and kaempferol, respectively, with the correlation r > 0.999 0. The extraction recoveries varied from 95% to 105%. Conclusion The method is accurate and selective, and can be used for the quality control of different parts of C. chinensis.
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[基金项目]
中药标准提取物的工业化色谱制备平台建设(2009ZX09301-012-105B)