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[摘要]
目的 对毛竹Phyllostachys edulis笋的化学成分进行研究.方法 利用反复硅胶柱色谱、开放性ODS柱色谱、Sephadex LH-20柱色谱和制备液相色谱等方法对其进行提取、分离和纯化,并结合核磁共振(NMR)、质谱等现代波谱技术对所得化合物进行结构鉴定.结果 从毛竹笋95%乙醇提取物中分离鉴定得到18个化合物,分别为脱氧胸腺嘧啶核苷(1)、尿嘧啶(2)、胸腺嘧啶(3)、香草酸(4)、对羟基苯甲醛(5)、腺嘌呤核苷(6)、尿嘧啶核苷(7)、2'-O-甲氧基尿嘧啶核苷(8)、对羟基苯甲酸乙酯(9)、环 (L-丙氨酸-L-缬氨酸)(10)、环 (L-苯丙氨酸-L-亮氨酸)(11)、酒渣碱(12)、ethyl 4-(sulfooxy) benzoate(13)、吲哚-3-甲酸甲酯(14)、顺式对羟基肉桂酸乙酯(15)、反式对羟基肉桂酸乙酯(16)、2-甲氧基-4-羟基-苯甲醛(17)、对羟基苯乙腈(18).结论 所有化合物均首次从毛竹笋中分得,化合物6~18为首次从刚竹属植物中分离得到.
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[Abstract]
Objective To investigate the chemical constituents from the shoots of Phyllostachys edulis. Methods Compounds were isolated by using a combination of various chromatographic techniques including silica gel, ODS, Sephadex LH-20, reversed-phase HPLC, and others. And the structures were elucidated by the nuclear magnetic resonance (NMR), mass spectrometry, and other modern spectroscopy. Results Eighteen compounds were isolated from the shoots of P. edulis and identified as thymidine (1), uracil (2), 5-ethyluracil (3), 4-hydroxy-3-methoxybenzoic acid (4), p-hydroxy-benzaldehyde (5), adenosine (6), uridine (7), 2'-O-methyluridine (8), ethyl-p-hydroxybenzoate (9), cyclo (L-Val-L-Ala) (10), cyclo (L-Phe-L-Leu) (11), flazine (12), ethyl 4-(sulfooxy) benzoate (13), 1H-indole-3-carboxylic acid, methyl ester (14), cis-p-hydroxyl ethyl cinnamate (15), trans-p-hydroxyl ethyl cinnamate (16), 4-hydroxy-2-methoxy-benzaldehyde (17), and methyl p-hydroxy benzeneacetate (18). Conclusion All the compounds are isolated from the shoots of P. edulis for the first time, and compounds 6—18 are firstly obtained from the plants of Phyllostachys Sieb. et Zucc.
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[基金项目]
国家自然科学基金资助项目(81160389)