目的 对青藏高原不同产地大花红景天Rhodiola crenulata中没食子酸、红景天苷、酪醇、儿茶素、没食子酸乙酯、对香豆酸进行分析研究,建立定量分析方法,为其进一步利用提供依据.方法 采用30%乙醇提取样品;Diamonsil C₁₈色谱柱(250 mm×4.6 mm,5 μm);以乙腈-0.3%磷酸溶液为流动相进行梯度洗脱;检测波长275 nm,柱温25 ℃.结果 没食子酸、红景天苷、酪醇、儿茶素、没食子酸乙酯、对香豆酸分别在38.2～382.0 ng/mL(r=0.999 8)、301.0～3 010.0 μg/mL(r=0.999 9)、19.8～198.0 ng/mL(r=0.999 6)、17.1～171.0 ng/mL(r=0.999 6)、4.5～45.0 ng/mL(r=0.999 8)、6.38～63.80 ng/mL(r=0.999 4)呈良好的线性关系,加样回收率均符合定量测定要求.结论 该方法简便,快速,准确,可用于同时对青藏高原大花红景天中6种活性成分的测定.

Objective To analyze the six active ingredients in Rhodiola crenulata from the different origins of Qinghai-Tibet Plateau, to establish quantitative analysis methods, and to provide a basis for further use. Methods Samples were extracted with 30% ethanol; In Diamonsil C₁₈ column (250 mm × 4.6 mm,5 μm), gradient elution was carried out with acetonitrile-0.3% phosphoric acid solution as the mobile phase, detection wavelength 275 nm, and column temperature 25 ℃. Results A good linear relationships of gallic acid, salidroside, tyrosol, catechin, ethyl gallate, and coumaric acid were at 38.2-382.0 (r = 0.999 8), 301.0-3 010.0 (r = 0.999 9), 19.8 -198.0 (r = 0.999 6), 17.1-171.0 (r = 0.999 6), 4.5-45.0 (r = 0.999 8), and 6.38-63.80 ng/mL (r = 0.999 4), respectively. Conclusion The method is simple, rapid, accurate, and can be used simultaneously to determine the content of the six active ingredients in R. crenulata from the different origins of Qinghai-Tibet Plateau.

"973"计划前期研究专项(2012CB722905)