[关键词]
[摘要]
目的 建立一种同时测定金沙藤中绿原酸、咖啡酸和p-香豆酰葡萄糖的方法.方法 采用HPLC法,色谱柱为Phenomenex Cl8(250 mm×4.6 mm,5 μm),甲醇-乙腈-1%冰醋酸(5:5:90)为流动相,检测波长为325 nm,柱温35 ℃.结果 绿原酸、咖啡酸和p-香豆酰葡萄糖分别在0.368~2.760、0.064~0.480、0.102~0.765 μg内呈良好的线性关系,平均加样回收率分别为101.48%、99.56%、101.73%,RSD分别为1.44%、0.92%、1.62%.结论 该方法操作简便、准确、重复性好,可用于金沙藤及其提取物的质量控制.
[Key word]
[Abstract]
Objective To establish a method for the determination of chlorogenic acid, caffeic acid, and 6-O-p-coumaroyl-D-glucopyranose in Lygodii Herba. Methods The determination was developed on Phenomenex C18 column (250 mm × 4.6 mm,5 μm), methanol-acetonitrile-1% glacial acetic acid (5︰5︰90) was used as mobile phase, the detective wavelength was 325 nm, and the column temperature was 35 ℃. Results The linear ranges of chlorogenic acid, caffeic acid, and 6-O-p-coumaroyl-D-glucopyranose were 0.368-2.760, 0.064-0.480, and 0.102-0.765 μg, respectively. The average recoveries were 101.48% (RSD = 1.44%), 99.56% (RSD = 0.92%), and 101.73% (RSD = 1.62%), respectively. Conclusion The method is simple, accurate, and highly reproducible, which could be used for the quality control of Lygodii Herba.
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[基金项目]
国家科技重大专项(2011ZX09201-201-17);西南民族药协同创新中心资助课题(CICSEM2013-A3);桂工信投资项目([2013]258)