目的 对25个薏苡种质资源进行序列相关扩增多态性(SRAP)分子标记研究.方法 采用改良CTAB法提取的薏苡叶片基因组DNA为模板,利用优化的SRAP-PCR反应体系,从88对引物中筛选出6对理想的引物组合,对25个薏苡种质资源进行SRAP-PCR扩增.结果 获得66条清晰可重复的多态性条带,多态率达93%,根据SRAP扩增结果,运用NTSYPC2.1分析软件计算各品种间的遗传相似系数,结果表明这些材料间的遗传相似系数在0.48～0.82.结论 聚类结果发现,25个薏苡居群可分为4个类群.

Objective To study the 25 germplasm resources of Coix lacryma-jobi by the technique of SRAP molecular markers. Methods Taking genome DNA in the leaves of C. lacryma-jobi extracted by modified CTAB method as template. Six ideal primer pairs were selected from 88 primer pairs by using the optimized SRAP-PCR reaction system and the amplification of 25 germplasm resources by SRAP-PCR were carried out. Results The total 66 clear and repeatable polymorphic bands were obtained, and the polymorphic rate was up to 93%. The coefficient of genetic similarity among each species was calculated by using NTSYPC 2.1 software. The results indicated that the coefficient of genetic similarity of these materials ranged from 0.48 to 0.82. Conclusion The 25 populations of C. lacryma-jobi could be clustered into four groups.

云南省科技厅基金项目(2011FB055);云南省科技计划项目(2014RE002)