目的 筛选米碎花Eurya chinensis 茎叶的微小核糖核酸(MicroRNAs,miRNAs)类药效物质及其靶基因功能分析.方法 采用高通量测序法,测定米碎花、正常小鼠和喂食米碎花匀浆液小鼠肝组织及血浆中miRNAs的表达丰度,并与GeneBank上的植物miRNAs数据库比对,结合实时荧光定量PCR(RTFQ-PCR)验证,鉴定吸收进入体内的米碎花miRNAs.以人类mRNA为靶标,采用TargetScan与MiRanda预测米碎花miRNAs的靶基因,并通过基因功能聚类(GO)与KEGG分析靶基因的相关功能.结果 共鉴定出5个米碎花miRNAs可吸收进入体内,其主要在生化过程、细胞成分和分子功能3大区域发挥作用,并影响肿瘤、代谢、炎症等相关信号通路.结论 miRNAs可能是一类新型的中药药效物质.

Objective To investigate the pharmacodynamic MicroRNAs (miRNAs) in Eurya chinensis and the functions of their target genes. Methods The miRNA profiles of E. chinensis in liver and plasma of normal mice and E. chinensis extract-treated mice were detected using high-flux sequencing, and then the miRNAs which took into the organism were validated by RTFQ-PCR using plant miRNA database in GeneBank. Human mRNA database as target was used to predict the target genes of miRNAs by TargetScan and MiRanda analysis, while the target gene functions were considered by GO and KEGG analyses. Results Five pharmacodynamic microRNAs were identified and took part in biological processes, cellular components, and molecular functions, which affected tumor, metabolism, and inflammation-related signal pathways. Conclusion The miRNAs might act as new pharmacodynamic materials of Chinese materia medica.

国家自然科学基金资助项目(81202977,81473339);浙江省卫生高层次创新人才项目