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[摘要]
目的 探讨消岩汤及其各拆方组分对人肺腺癌A549细胞株凋亡的影响及其机制.方法 将消岩汤按照功效进行拆方,分为清热解毒组、活血化瘀组、益气扶正组.以CCK-8法检测各拆方对A549细胞的最有效抑制浓度,采用Western blotting法测定Survivin、Caspase-3蛋白表达,RT-PCR法检测survivin与caspase-3基因表达.结果 消岩汤及其各拆方组分均有明显的抑制A549细胞生长作用,消岩汤及其各拆方组分具有诱导A549细胞凋亡的作用,其机制与下调survivin基因和蛋白表达有关,对caspase-3基因和蛋白表达影响不显著.结论 消岩汤及其各拆方组分体外均有明显的抑制A549细胞生长作用,消岩汤及其各拆方组分可通过下调survivin的表达诱导肿瘤细胞凋亡,清热解毒组方中药是其抗肿瘤作用主要组分.
[Key word]
[Abstract]
Objective To investigate the inhibition of Xiaoyan Decoction and its decomposed recipe on apoptosis of pulmonary carcinosis A549 cells. Methods According to the effect, Xiaoyan Decoction is divided into three groups:Qingre Jiedu (clearing away heat-evil and expelling superficial evils) group, Huoxue Huayu group, and Yiqi Fuzheng group. Using CCK 8 method to detect the proliferation activity of lung adenocarcinoma cancer A549 cells. The Western blotting was adopted to observe the influence on protein expression of survivin and caspase-3 of pulmonary carcinosis A549 cells. And the RT-PCR was adopted to observe the influence on survivin and caspase-3 gene expression of pulmonary carcinosis A549 cells. The inhibition of Xiaoyan Decoction and its decomposed recipe on the apoptosis of pulmonary carcinosis A549 cells was investigated. Results There were obvious inhibitory effects of cancer cell activity in the three groups. Xiaoyan Decoction in the three groups could induce the apoptosis of lung cancer A549 cell line. Its mechanism was the down regulation of survivin expression. And the related with the elevation of caspase-3 expression is not intimate. Conclusion Xiaoyan Decoction has the antitumor effect by inducing tumor cell apoptosis through the down regulation of survivin expression.
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[基金项目]
天津市教委课题"消岩汤对肺腺癌A549细胞凋亡生成素基因表达的影响"(20120216)