目的 对生品肉豆蔻Myristicae Semen的化学成分进行研究.方法 采用硅胶柱色谱、Sephadex LH-20柱色谱和制备型HPLC等方法进行分离纯化,结合波谱数据分析鉴定化合物结构.结果 从肉豆蔻75%醇提物的醋酸乙酯萃取物中分离得到18个化合物,分别鉴定为香草酸(1)、紫铆因(2)、(2R)-3-(3', 4', 5'-三甲氧基苯基)-1, 2-丙二醇(3)、硫磺菊素(4)、3-甲氧基-4, 5-亚甲二氧基肉桂酸(5)、7, 3', 4'-三羟基黄酮(6)、7-羟基色原酮(7)、verrucosin(8)、(+)-赤-(7S, 8R)-Δ^8'-7-羟基-3, 4, 3', 5'-四甲氧基-8-氧代-4'-新木脂素(9)、(-)-赤-(7R, 8S)-Δ^8'-7-乙酰基-3, 4, 3', 5'-四甲氧基-8-氧代-4'-新木脂素(10)、nectandrin B(11)、(-)-(7S, 7'R, 8S, 8'R)-4, 4'-二羟基-3, 5, 3'-三甲氧基-7, 7'-环氧木脂素(12)、fragransin B₃(13)、fragransin B₁(14)、(-)-enantiomer(15)、(-)-赤-(7R, 8S)-Δ^8'-7-羟基-3, 4, 5, 3', 5'-五甲氧基-8-氧代-4'-新木脂素(16)、(+)-赤-(7S, 8R)-Δ^8'-7, 4-二羟基-3, 5, 3', 5'-四甲氧基-8-氧代-4'-新木脂素(17)、(+)-5-甲氧基脱氢二异丁香酚(18).结论 化合物2、4～7为从肉豆蔻属植物中首次分离得到,化合物1为从该植物中首次分离得到.

Objective To study the chemical constituents in the dry mature seeds of Myristica fragrans (Myristicae Semen). Methods Compounds were isolated and purified from the ethyl acetate layer of 75% alcohol extract by means of the silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative HPLC isolation methods. Compound structures were identified by analyzing and comparing the spectral data with those for them in the reference. Results Eighteen compounds were obtained from the ethyl acetate layer of 75% ethanol extract in Myristicae Semen. On the basis of spectral data and combined with the references, these compounds had been identifited as:vanilic acid (1), butein (2), (2R)-3-(3', 4', 5'-trimethoxyphenyl)-1, 2-propanediol (3), sulphuretin (4), 3-methoxy-4, 5-methylenedioxy-cinnamic acid (5), 7, 3', 4'-trihydroxyflavanone (6), 7-hydroxy-4-benzopyrone (7), verrucosin (8), (+)-erythro-(7S, 8R)-Δ^8'-7-hydroxy-3, 4, 3', 5'-tetramethoxy-8-O-4'-neolignan (9), (-)-erythro-(7R, 8S)-Δ^8'-7-acetoxy-3, 4, 3', 5'-tetramethoxy-8-O-4'-neolignan (10), nectandrin B (11), (-)-(7S, 7'R, 8S, 8'R)-4, 4'-dihydroxy-3, 5, 3'-trimethoxy-7, 7'-epoxylignan (12), fragransin B₃ (13), fragransin B₁ (14), (-)-enantiomer (15), (-)-erythro-(7R, 8S)-Δ^8'-7-hydroxy-3, 4, 5, 3', 5'-pentamethoxy-8-O-4'-neolignan (16), (+)-erythro-(7S, 8R)-Δ^8'-7, 4-dihydroxy-3, 5, 3', 5'-tetramethoxy-8-O-4'-neolignan (17), and (+)-5-methoxydeydrodiisoeugenol (18). Conclusion Compounds 2 and 4－7 are isolated from the plants of Myristica Gronov. for the first time. Compound 1 is isolated from this plant for the first time.

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