目的 研究马兰Kalimeris indica全草的化学成分。方法 采用硅胶柱色谱、Sephadex LH-20柱色谱等方法分离纯化,根据理化性质和波谱数据进行结构鉴定。结果 从马兰全草95%乙醇中分离得到了15个化合物,分别鉴定为木栓酮(1)、表木栓醇(2)、豆甾醇(3)、β-谷甾醇(4)、α-菠甾醇(5)、古柯二醇(6)、十八烷酸(7)、梣酮(8)、β-胡萝卜苷(9)、二十六烷醇(10)、麦角甾醇(11)、牛防风素(12)、邻苯二甲酸二丁酯(13)、羽扇豆醇乙酸酯(14)、达玛二烯醇乙酸酯(15)。结论 化合物5、8～13均为该植物中首次分离得到。化合物1、3、5、8、12对苹果腐烂病菌和水稻纹枯病菌均有抑制作用。

Objective To study the chemical constituents from Kalimeris indica. Methods Chemical constituents were isolated by column chromatography and semi-prepared HPLC, and the structures were elucidated by spectral data and physical-chemical properties. Results Fifthteen compounds were isolated and respectively identified as friedelin (1), epifriedelanol (2), stimasterol (3), β-sitosterol (4), α-spinasterol (5), erythodiol (6), octadecanoic acid (7), fraxinellone (8), β-daucosterol (9), hexacosanol (10), ergoaterol (11), sphondin (12), dibntyl phthalate (13), oleanic acid (14), and dammerdienly acetate (15). Conclusion Compounds 5, 8—13 are isolated from K. indica for the frist time. Compounds 1, 3, 5, 8, and 12 have inhibitory effects on Cytospora sp. and Rhizoctonia solani.

黑龙江省自然科学基金资助项目(B201314);黑龙江省教育厅资助项目(12521595)