[关键词]
[摘要]
目的 比较白芍与赤芍的化学组成差异,为其质量控制提供研究依据。方法 采用1H-NMR代谢组学技术对白芍与赤芍进行分析,通过多元统计方法找出其化学差异成分,并分析差异成分之间的相关性。结果 白芍与赤芍核磁共振指纹图谱中共指认出了代谢物32种,多元统计分析显示白芍与赤芍可明显区分,白芍中精氨酸、苏氨酸、乙酸、天冬氨酸、谷氨酰胺、γ-氨基丁酸、柠檬酸、丁二酸、乳酸、芍药内酯苷、6-O-没食子酰白芍苷、1, 2, 3, 4, 6-五没食子酰基葡萄糖以及没食子酸的量较高,而赤芍中丙氨酸、α-葡萄糖、蔗糖、芍药苷、儿茶素、β-谷甾醇、脂肪酸以及丹皮酚的量较高。此外,二者差异成分之间的相关系数也存在较大差异。结论 本研究从整体化学组成上比较了白芍与赤芍的差异,不仅为白芍、赤芍质量标准研究提供了一种新思路,而且为其功效与化学成分的相关性研究提供依据。
[Key word]
[Abstract]
Objective To compare the chemical constituents of Paeoniae Alba Radix and Paeoniae Rubra Radix, and to provide a basis for quality evaluation. Methods NMR-based metabolomic approach combined with multivariate statistical analysis was used to investigate the differential metabolites between Paeoniae Alba Radix and Paeoniae Rubra Radix. Results Thirty-two metabolites were identified in the 1H-NMR spectra, and the multivariate statistical analysis showed that Paeoniae Alba Radix and Paeoniae Rubra Radix could be separated clearly. Paeoniae Alba Radix contained more arginine, threonine, acetic acid, aspartic acid, glutamine, GABA, citric acid, succinate, lactate, albiflorin, 6-O-galloyl albiflorin, 1, 2, 3, 4, 6-pentagalloyglucose, and gallic acid, while Paeoniae Rubra Radix contained more alanine, α-glucose, sucrose, paeoniflorin, catechin, β-sitosterol, fatty acid, and paeonol. In addition, the Pearson correlations between differential metabolites of Paeoniae Alba Radix and Paeoniae Rubra Radix also showed apparent differences. Conclusion The results reveal the chemical differences between Paeoniae Alba Radix and Paeoniae Rubra Radix in a holistic way, and provide a scientific basis for assessing the quality of Paeoniae Alba Radix and Paeoniae Rubra Radix, as well as the correlations between the chemical constituents and pharmacological efficacy.
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[基金项目]
山西省科技创新重点团队(2013131015);山西省高等学校创新人才支持计划