目的 建立高效液相色谱法同时测定不同种质资源金银花中新绿原酸、绿原酸、隐绿原酸、咖啡酸、3, 4-二咖啡酰奎尼酸、3, 5-二咖啡酰奎尼酸和4, 5-二咖啡酰奎尼酸等7种有机酸及断氧马钱子苷的量。方法 采用高效液相色谱法测定，色谱条件为phenomenex C₁₈色谱柱（250 mm×4.6 mm，5 μm），流动相0.1% 磷酸溶液和甲醇采用梯度洗脱，检测波长225 nm，体积流量0.8 mL/min。结果 金银花中新绿原酸、绿原酸、隐绿原酸、咖啡酸、3, 4-二咖啡酰奎尼酸、3, 5-二咖啡酰奎尼酸和4, 5-二咖啡酰奎尼酸及断氧马钱子苷在各自的线性范围内均呈良好的线性关系，平均加样回收率分别为99.41%、99.05%、99.01%、98.71%、98.79%、98.82%、98.89%、99.16%，RSD分别为0.56%、0.37%、0.93%、1.11%、0.90%、0.87%、0.71%、1.04%。结论 该方法快速、简便、准确，可用于不同种质金银花中7种有机酸及断氧马钱子苷的定量测定。

Objective To determine and compare the contents of seven kinds of organic acids (neochlorogenic acid, chlorogenic acid, 4-dicaffeoylquinicacid, caffeic acid, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid, and 4, 5-dicaffeoylquinic acid) and secoxyloganin in Lonicerae Flos. Methods The contents were determined by HPLC. The analysis was carried out with gradient elution on a Phenomenex C₁₈ colunm eluted with methanol and 0.1% phosphoric acid. The detection wavelength was set at 225 nm. Results Neochlorogenic acid, chlorogenic acid, 4-dicaffeoylquinicacid, caffeic acid, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid, and 4, 5-dicaffeoylquinic acid in Lonicerae Flos and secoxyloganin in their linear ranges showed good linear relationship, the average recoveries (n = 9) were 99.41%, 99.05%, 99.01%, 98.71%, 98.79%, 98.82%, 98.89%, and 99.16%, and RSD values were 0.56%, 0.37%, 0.93%, 1.11%, 0.90%, 0.87%, 0.71%, and 1.04%. Conclusion The method is rapid, simple, accurate, and can be used for quality evaluation of the seven kinds of organic acids in Lonicerae Flos from different germplasm and secoxyloganin.

科技部重大新药创制：现代中药创新集群与数字制药技术平台（2013ZX09402203）