[关键词]
[摘要]
目的 研究西藏产齿叶玄参Scrophularia dentata干燥全草的低极性化学成分。方法 利用溶剂提取和正、反相硅胶柱色谱,Sephadex LH-20凝胶柱色谱等技术进行分离纯化,并通过MS、NMR波谱技术鉴定化合物结构。结果 从西藏产齿叶玄参干燥全草95%乙醇提取物中分离得到15个低极性化合物,分别鉴定为齐墩果酸(1)、乙酰齐墩果酸(2)、豆甾- 4- 烯-3-酮(3)、豆甾-4, 22-二烯-3-酮(4)、2, 6-二甲氧基苯醌(5)、酵母甾醇(6)、肉桂酸(7)、菠菜甾醇(8)、β-谷甾醇(9)、2-三十三烷酮(10)、2-(4-hydroxyphen-yl)-ethyl 1-dodecyloctadecanoate(11)、芹菜素(12)、金圣草黄素(13)、杜仲树脂酚(14)、丁香脂素(15)。结论 所有化合物均为首次从该植物中分离得到,化合物2~6、10~15为首次从该属植物中分离得到。
[Key word]
[Abstract]
Objective To study the low-polarity chemical constituents from the whole plants of Scrophularia dentata grown in Tibet. Methods The compounds were isolated and purified by various column chromatography. Their structures were elucidated by means of spectral analyses (MS, 1H-NMR, and 13C-NMR). Results Fifteen compounds were isolated and identified as oleanolic acid (1), 3-O-acetyloleanolic acid (2), stigmast-4-en-3-one (3), stigmast-4, 22-dien-3-one (4), 2, 6-dimethoxybenzoquinone (5), cerevisterol (6), cinnamic acid (7), spinasterol (8), β-sitosterol (9), 2-tritriacontanone (10), 2-(4-hydroxyphen-yl)-ethyl 1-dodecyloctadecanoate (11), apigenin (12), chrysoeriol (13), medioresinol (14), and syringaresinol (15). Conclusion All the compounds are firstly isolated from this plant and compounds 2—6 and 10—15 are isolated from the plants of this genus for the first time.
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[基金项目]
国家自然科学基金资助项目(81173518);上海市教委“东方学者”跟踪计划;上海高校青年教师培养资助计划(ZZszy13057);上海中医药大学预算内项目(2013JW11)