目的 阐明二神丸中药物炮制后（盐炙补骨脂+麸煨肉豆蔻）其不同提取部位的指纹图谱特征峰所代表的化学成分及其对温脾止泻药效贡献的大小，表征二神丸的药效物质基础。方法 采用HPLC法建立二神丸不同提取部位的指纹图谱，以不同提取部位对番泻叶所致急性腹泻小鼠腹泻指数的影响研究其止泻作用，采用灰关联分析法建立其谱效关系。结果 建立了二神丸不同提取部位的指纹图谱，相似度均大于0.90；二神丸温脾止泻的药效是多种成分共同作用的结果，各特征峰所代表的化学成分对其止泻药效贡献的大小顺序（按特征峰编号）为6号>18号>7号>20号>14号>21号>1号>25号>24号>23号>4号>3号>8号>11号。结论 所构建的二神丸不同提取部位HPLC指纹图谱方法简单，重复性好，可为二神丸的质量评价提供参考；不同部位指纹图谱与其止泻药效之间有一定的对应关系，为进一步研究二神丸的炮制机制奠定了基础。

Objective To clarify the contribution of the fingerprint peaks from different parts of prepared Ershen Pill to the effect of warming spleen to relieve diarrhea, and to reflect the material basis. Methods The HPLC fingerprints of different parts of Ershen Pill (composed of Psoraleae Fructus stir-baked with saltwater and Myristica Fragrans simmered with bran) were established. The diarrhea index of acute diarrhea in mice caused by senna leaf was researched to compare the effect of warming spleen to relieve diarrhea. Results The fingerprints of different parts of Ershen Pill were established, and all of the similarity degrees were over 0.90. The effect of warming spleen to relieve diarrhea of Ershen Pill was obtained from many constituents collaboratively. The contribution of different peaks to relieve diarrhea with astringents was ranked as 6 > 18 > 7 > 20 > 14 > 21 > 1 > 25 > 24 > 23 > 4 > 3 > 8 > 11. Conclusion The method for establishing HPLC fingerprints of different parts of Ershen Pill is simple and repeatable. The relationship between the fingerprints and the effect of warming spleen to relieve diarrhea is paralleled to some extent. And this will lay a foundation for the research of processing mechanism of Ershen Pill.

国家自然科学基金资助项目（81274085）