目的 探讨红景天苷对支气管哮喘小鼠气道重塑及核因子-κB(NF-κB)和转化生长因子-β1(TGF-β1)信号通路的影响。方法 40只清洁级雌性BABL/c小鼠，随机分为4组，每组10只，分别为对照组、模型组、红景天苷低剂量组、红景天苷高剂量组。以卵蛋白(OVA)致敏、激发制备小鼠哮喘模型，在末次激发24 h后取小鼠左肺组织行苏木精-伊红(HE)染色、PAS染色、Masson染色；取右肺组织分别用酶联免疫吸附法(ELISA)、RT-PCR和Western blotting检测支气管肺泡灌洗液(BALF)中白细胞介素(IL)-4、IL-5、IL-13水平以及TGF-β1 mRNA、蛋白和NF-κB p65蛋白表达。结果 模型组与对照组比较，小鼠BALF中炎症细胞计数、IL-4、IL-5、IL-13水平增高；肺组织TGF-β1 mRNA、蛋白和NF-κB p65蛋白表达水平均显著升高(P<0.05、0.01)。红景天苷组与模型组比较，小鼠BALF中炎症细胞计数、IL-4、IL-5、IL-13水平，TGF-β1 mRNA、蛋白和NF-κB p65蛋白表达水平均显著降低(P<0.05、0.01)，不同剂量红景天苷组间上述各指标间差异无统计学意义(P>0.05)。结论 红景天苷可抑制哮喘小鼠气道重塑的发生，其部分机制可能是通过抑制NF-κB/TGF-β1信号通路而实现的。

Objective To investigate the effects of salidroside on airway remodeling and NF-κB and TGF-β1 signal transduction pathway in asthmatic mice. Methods Forty female BALB/c mice of clean grade were randomly divided into four groups (n=10), such as control, egg protein (OVA) asthma, low-, and high-dose salidroside groups. OVA was used to establish the asthmatic mice model, and the left lung tissues of all mice were treated by HE, PAS, and Masson trichrome staining after the last excitation in 24 h. The right lung tissues were detected by enzyme linked immunosorbent assay (ELISA) to observe the expression levels of IL-4, IL-5, and IL-13 in bronchoalveolar lavage fluid (BALF), by RT-PCR to investigate the expression level of TGF-β1 mRNA, and by Western blotting to detect the expression levels of TGF-β1 and NF-κB p65 proteins. Results Compared with the control group, the inflammatory cell counts and the expression levels of IL-4, IL-5, and IL-13 in asthmatic model group were increased. The expression levels of TGF-β1 mRNA and NF-κB protein from lung tissues were significantly higher than those in the control group (P < 0.01). Compared with the asthma model group, the inflammatory cell counts, the expression levels of IL-4, IL-5, IL-13 in BALF, TGF-β1 mRNA, and NF-κB of p65 protein were significantly decreased in the salidroside intervention groups, with significant differences (P < 0.01). But for indexes mentioned above, there were no statistical significances between the two different treatment groups (P > 0.05). Conclusion Salidroside might inhibit the development of airway remodeling in asthmatic mice, and the mechanism might be partly achieved through the inhibition of NF-κB/TGF-β1 signal transduction pathway.

国家自然科学基金资助项目（81260665）；吉林省科技厅中青年科技创新领军人才及创新团队项目（20140519013JH）；吉林省教育厅重点项目（吉教科合字2013第2号）